PXD057920-1

PXD057920 is an original dataset announced via ProteomeXchange.

Title	Proteomic insight into the ontogeny of blood-meal digestion in the tick Ixodes ricinus
Description	The long blood feeding duration of hard ticks relies on the continuous modulation of saliva composition to manipulate host haemostatic and immune systems. While the exogenous muscarinic acetylcholine receptor (mAChR) agonist, pilocarpine, is a potent inducer of tick salivation, the underlying control mechanisms of endogenous stimulation remain unclear. Here, we characterised two pharmacologically distinct types of mAChRs (A and B) in the genome of the Ixodes ricinus tick. A combination of molecular dynamics and directed mutagenesis revealed the atypical muscarinic profile of the type-B receptor. Immunolabelling followed by in vivo pharmacology and proteomic analyses suggested that the composition of secreted saliva is a result of a complex interplay between different central neurons and specific regions of the salivary gland (SG), mediated by distinct axonal mAChR types. This system likely functions upstream of a neuropeptide signalling cascade, ultimately controlling different salivation subunits in SG. Our study describes a unique model of regulation of tick SG activity in a medically relevant tick species. This knowledge holds broader implications for understanding tick-host interactions.
HostingRepository	PRIDE
AnnounceDate	2025-10-07
AnnouncementXML	Submission_2025-10-06_18:05:44.249.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Filip Dyčka
SpeciesList	scientific name: Ixodes ricinus; NCBI TaxID: NEWT:34613;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-14 22:51:32	ID requested
⏵ 1	2025-10-06 18:05:45	announced

Kozelkov, á T, Horn M, Sojka D, Lu S, Pytelkov, á J, Urbanov, á V, Dy, č, ka F, Mare, š M, Kop, á, č, ek P, Proteomic Insight Into the Ontogeny of Blood-Meal Digestion in the Tick Ixodes ricinus. Mol Cell Proteomics, 24(9):101054(2025) [pubmed]

10.1016/j.mcpro.2025.101054;

submitter keyword: LC-MSMS, midgut, proteomics, tick,Ixodes ricinus, adult

Petr Kopáček
contact affiliation	Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, Branisovska 1160/31, 37005 Ceske Budejovice, Czech Republic
contact email	kopajz@paru.cas.cz
lab head
Filip Dyčka
contact affiliation	University of South Bohemia in Ceske Budejovice Faculty of Science Branisovska 1760 Ceske Budejovice
contact email	dycka@mail.muni.cz
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/10/PXD057920

PRIDE project URI

• PRIDE
  1. PXD057920
     1. Label: PRIDE project
     2. Name: Proteomic insight into the ontogeny of blood-meal digestion in the tick Ixodes ricinus