PXD057907-1

PXD057907 is an original dataset announced via ProteomeXchange.

Title	Profiling proteome responses to auxin transport associated with sugarcane somatic embryo development
Description	Sugarcane is a crop widely used worldwide for bioenergy production, and its genetic breeding is highly dependent on tissue culture techniques. Auxin biosynthesis and transport are critical processes for in vitro tissue culture development, but the regulatory responses associated with these processes are still poorly understood in plants. We investigated the role of auxin transport during somatic embryo differentiation in sugarcane callus using an auxin transport inhibitor through a high-throughput proteomic approach. Four concentrations of 2,3,5-triiodobenzoic acid (TIBA 0, 1, 10, and 100 µM) were tested on sugarcane embryogenic callus during the pre-maturation phase of somatic embryogenesis. Pre-maturation treatment with 100 µM TIBA inhibited the differentiation of embryogenic callus into somatic embryos. Then, embryogenic callus from control and 100 µM TIBA treatments were compared via proteomic analysis. A total of 1221 proteins were found, of which 740 were differentially accumulated (DAP) in the 100 µM TIBA/Control comparison. Among the DAPs, 33 were unique to the control treatment, 31 were unique to the 100 µM TIBA pre-maturation treatment, and 415 down-regulated and 261 up-regulated. Inhibition of auxin transport impaired the accumulation and predicted protein‒protein interactions involved in embryonic development, translation, ubiquitination, and energy metabolism. The levels of spermidine synthase and spermidine were reduced, suggesting an association between auxin and polyamines during embryonic development in vitro. The down-regulation of the proteins chaperone BOBBER1, Cullin-associated NEDD8-dissociated protein 1, and Receptor-like kinase TMK2 could be associated with auxin responses and the predicted interactions between proteasome members found during the differentiation of somatic embryos. These new findings demonstrate the role of auxin transport in downstream responses during somatic embryo differentiation and open new avenues for exploring the potential of sugarcane for biotechnological purposes.
HostingRepository	PRIDE
AnnounceDate	2025-09-19
AnnouncementXML	Submission_2025-09-19_10:21:26.155.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Vanildo Silveira
SpeciesList	scientific name: Saccharum hybrid cultivar SP80-3280; NCBI TaxID: 193079;
ModificationList	No PTMs are included in the dataset
Instrument	SYNAPT G2-Si

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-14 15:09:48	ID requested
⏵ 1	2025-09-19 10:21:26	announced

10.1007/S40626-025-00375-2;

submitter keyword: Auxin transport

Embryo development

Plant morphogenesis

Comparative proteomics.

Vanildo Silveira
contact affiliation	Laboratório de Biotecnologia, Centro de Biociências e Biotecnologia (CBB), Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Av. Alberto Lamego, Campos dos Goytacazes, RJ, Brazil. Unidade de Biologia Integrativa, Setor de Genômica e Proteômica, UENF, Campos dos Goytacazes, RJ, Brazil.
contact email	vanildo@uenf.br
lab head
Vanildo Silveira
contact affiliation	UNIVERSIDADE ESTADUAL DO NORTE FLUMINENSE
contact email	vanildo@uenf.br
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/09/PXD057907

PRIDE project URI

• PRIDE
  1. PXD057907
     1. Label: PRIDE project
     2. Name: Profiling proteome responses to auxin transport associated with sugarcane somatic embryo development