PXD057906-1

PXD057906 is an original dataset announced via ProteomeXchange.

Title	Stress granule phase separation in stress-responsive cytosolic extract-in-oil droplets
Description	Stress granules (SGs) are biomolecular condensates that transiently form in the cytosol when cells are exposed to potentially hazardous conditions. Illustrating the physiological relevance of SGs, dysregulation of pathways leading to their assembly or disassembly is increasingly associated with human diseases related to ageing and neurodegeneration. In this study, we have devised a novel methodology for studying and manipulating SG phase separation in vitro, which offers a more sophisticated level of complexity. This innovative stress-responsive methodology empolyed emulsion-based droplet microfluidics to recreate an in vitro environment that resembles the confined active cytosolic milieu. After using different stress triggers, a condensate composition analysis by quantitative LC-MS/MS identified new SG client proteins.
HostingRepository	PRIDE
AnnounceDate	2026-05-19
AnnouncementXML	Submission_2026-05-19_09:11:37.363.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Pietro Scaturro
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	acetylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-14 11:11:39	ID requested
⏵ 1	2026-05-19 09:11:37	announced

Dataset with its publication pending

submitter keyword: droplet-based, integrated stress response, microfluidics, dsRNA, biomolecular condensates, G3BP1,Stress granules, liquid-liquid phase separation

Pietro Scaturro
contact affiliation	Systems Arbovirology lab, Leibniz Institute of Virology, Hamburg, Germany
contact email	pietro.scaturro@leibniz-liv.de
lab head
Pietro Scaturro
contact affiliation	Systems Arbovirology, Leibniz Institute of Virology
contact email	pietro.scaturro@tum.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD057906

PRIDE project URI

• PRIDE
  1. PXD057906
     1. Label: PRIDE project
     2. Name: Stress granule phase separation in stress-responsive cytosolic extract-in-oil droplets