PXD057885-1

PXD057885 is an original dataset announced via ProteomeXchange.

Title	LC-MS/MS analysis of HD5-interacting proteins
Description	Human enteric α-defensin 5 (HD5) acts as a “double-edged sword” in host immunity due to its prominent role in promoting pathogen infections, yet the mechanistic basis of its infection-enhancing operation remains unclarified. Here we show that HD5 induces abundant filopodial extensions in epithelial cells which capture Shigella, a major human enteroinvasive pathogen that exploits filopodia for invasion, at high efficiency, suggesting a novel human-specific mechanism of HD5-augmented bacterial invasion. Using multi-omics screening and both gut-on-chip and in vivo models, we identify the HD5 receptor as P2Y11, a purinergic receptor distributed apically on the luminal surface of human colonic epithelium, which to our knowledge is the first HD5 receptor for a specific cellular function. cAMP-PKA signaling was identified to be the main pathway mediating the cytoskeleton-regulating activity of HD5, whose critical residues involved were also determined. In illuminating this mechanism of Shigella invasion, our findings raise the possibility of new intervention strategies against HD5-augmented infections.
HostingRepository	PRIDE
AnnounceDate	2024-12-16
AnnouncementXML	Submission_2024-12-15_22:32:24.005.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mengyao Guo
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-14 01:44:48	ID requested
⏵ 1	2024-12-15 22:32:24	announced

10.1038/S41564-024-01901-9;

submitter keyword: Human,LC-MS/MS,HD5

Dan Xu
contact affiliation	Xi'an Jiaotong University
contact email	dan.xu@xjtu.edu.cn
lab head
Mengyao Guo
contact affiliation	Xi'an Jiaotong University
contact email	myguo123@stu.xjtu.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/12/PXD057885

PRIDE project URI

• PRIDE
  1. PXD057885
     1. Label: PRIDE project
     2. Name: LC-MS/MS analysis of HD5-interacting proteins