PXD057883-1

PXD057883 is an original dataset announced via ProteomeXchange.

Title	A Comprehensive Comparison of Sample Preparation and Normalization Methods for Bottom-Up Proteomics of Extracellular Vesicles
Description	In this scientific study, we evaluated sample preparation methods for exosome proteomics along with data normalization techniques. We commenced by extracting exosomal proteins using three different lysis buffers: 8M urea, 1% sodium deoxycholate (SDC), and 0.1% sodium dodecyl sulfate (SDS). Through comparative analysis, we determined that 1% SDC was the most efficient lysis buffer, yielding the highest quality and quantity of protein extracts. Subsequently, we examined various data normalization methods to ensure reliable and reproducible results in proteomic profiling. Our findings highlight the critical role of optimal sample preparation and data normalization in exosome proteomics, which are essential for accurate biomarker discovery and understanding exosomal functions in biological processes. This research provides a foundation for standardized protocols in exosome proteomics and contributes to the enhancement of data quality in this burgeoning field.
HostingRepository	iProX
AnnounceDate	2024-11-14
AnnouncementXML	Submission_2024-11-13_23:31:06.549.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jun Wang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-13 23:30:07	ID requested
⏵ 1	2024-11-13 23:31:06	announced

Dataset with its publication pending

submitter keyword: extracellular vesicles, sample preparation, lysate buffer, normalization

Haojie Lu
contact affiliation	Fudan University
contact email	luhaojie@fudan.edu.cn
lab head
Jun Wang
contact affiliation	Fudan University
contact email	21110220037@m.fudan.edu.cn
dataset submitter

iProX dataset URI