PXD057797-1

PXD057797 is an original dataset announced via ProteomeXchange.

Title	Mechanisms of recalcitrant fucoidan breakdown in marine Planctomycetota
Description	Marine brown algae produce the highly recalcitrant polysaccharide fucoidan, contributing to long-term oceanic carbon storage and climate regulation. Fucoidan is degraded by specialized heterotrophic bacteria, which promote ecosystem function and global carbon turnover using largely uncharacterized mechanisms. Here, we isolate and study two Planctomycetota strains from the microbiome associated with the alga Fucus spiralis, which grow efficiently on chemically diverse fucoidans. One of the strains appears to internalize the polymer, while the other strain degrades it extracellularly. Multi-omic approaches show that fucoidan breakdown is mediated by the expression of divergent polysaccharide utilization loci, and endo-fucanases of family GH168 are strongly upregulated during fucoidan digestion. Enzymatic assays and structural biology studies reveal how GH168 endo-fucanases degrade various fucoidan cores from brown algae, assisted by auxiliary hydrolytic enzymes. Overall, our results provide insights into fucoidan processing mechanisms in macroalgal-associated bacteria.
HostingRepository	PRIDE
AnnounceDate	2024-11-22
AnnouncementXML	Submission_2024-11-22_01:21:20.293.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mikel Azkargorta
SpeciesList	scientific name: Planctomycetales; NCBI TaxID: 112;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-12 07:19:23	ID requested
⏵ 1	2024-11-22 01:21:20	announced

Dataset with its publication pending

submitter keyword: Fucoidan breakdown, carbon sequestration, macroalgal-associated bacteria, endo-fucanase GH168, Planctomycetota

Felix Elortza
contact affiliation	Proteomics Platform CIC bioGUNE, Bizkaia Tech. Park Build. 800, 48160 (Derio) Spain
contact email	felortza@cicbiogune.es
lab head
Mikel Azkargorta
contact affiliation	Proteomics Platform CIC bioGUNE
contact email	mazkargorta@cicbiogune.es
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD057797
     1. Label: PRIDE project
     2. Name: Mechanisms of recalcitrant fucoidan breakdown in marine Planctomycetota