PXD057751-1

PXD057751 is an original dataset announced via ProteomeXchange.

Title	The proteomic and peptidomic response of wheat (Triticum aestivum L.) to drought stress
Description	Drought negatively impacts the morphological, physiological, and molecular characteristics of plants. Here we performed quantitative proteomic and peptidomic analysis of spring wheat (Triticum aestivum L.). leaves and roots under drought stress conditions. Using isobaric tags for relative and absolute quantitation (iTRAQ), we identified 497 and 157 differentially expressed (DE) proteins in leaves and roots, respectively. The upregulated DE proteins in leaves were primarily involved in stress responses, such as oxidative stress and heat response, while those in roots were associated with metabolic processes like UDP-glucose metabolism and glutamine biosynthesis. Using peptidomic approaches, we identified 2294 endogenous extracellular peptides, including members of 16 small secreted peptides (SSPs) families, in the root secretome. In the stress-induced secretomes, we identified endogenous peptides, derived from proteins involved in cell wall catabolism and detoxification and involved in intercellular signaling and stress response. Identification of proteins and secretome peptides regulating the drought stress response helps to understand adaptation mechanisms and develop new agricultural technologies to increase productivity.
HostingRepository	PRIDE
AnnounceDate	2025-08-04
AnnouncementXML	Submission_2025-08-03_16:09:56.623.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Igor Fesenko
SpeciesList	scientific name: Triticum aestivum (Wheat); NCBI TaxID: 4565;
ModificationList	iTRAQ8plex-116 reporter+balance reagent acylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-11 07:41:37	ID requested
⏵ 1	2025-08-03 16:09:57	announced

10.3390/plants14142168;

Azarkina R, Makeeva A, Mamaeva A, Kovalchuk S, Ganaeva D, Tikhonovich I, Fesenko I, L.) to Drought Stress. Plants (Basel), 14(14):(2025) [pubmed]

submitter keyword: Spring wheat “Leningradskaya 6” cultivar, proteome, peptidome, iTRAQ, secretome, drought, LC-MS/MS

Regina Azarkina
contact affiliation	Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Moscow, Russia
contact email	khazigaleeva.regina@gmail.com
lab head
Igor Fesenko
contact affiliation	IBCH RAS
contact email	fesigor@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD057751
     1. Label: PRIDE project
     2. Name: The proteomic and peptidomic response of wheat (Triticum aestivum L.) to drought stress