⮝ Full datasets listing
PXD057714-1
PXD057714 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Proteomic analysis of Cdkal1-KO mouse podocyte cell line |
| Description | E11 murine kidney podocyte cell line was purchased from Cell Lines Service. Cells were cultured in RPMI medium (Wako) supplemented with 10% fetal bovine serum (Sigma) at 33°C and induced to differentiate by culture at 37°C for two weeks and 5% CO2. Cdkal1 knockout in the E11 cell line was performed using a guide RNA targeting exon 5 of Cdkal1 followed by cloning into the Lenti-CRISPR v2 vector (Addgene). The cell lysates were digested by trypsin by phase-transfer surfactant method. The digested samples were desalted and reconstituted with 0.1% trifluoroacetic acid after drying. The digested peptide samples corresponding to 1 ug protein were injected into liquid chromatography-tandem mass spectrometry. The data acquired by SWATH modes on TripleTOF6600 (SCIEX, Framingham, MA, USA) with the Eksigent NanoLC400 system (SCIEX). Proteins were identified and quantified with DIA-NN 1.8 with UniProt Human reference proteome data. |
| HostingRepository | jPOST |
| AnnounceDate | 2025-11-11 |
| AnnouncementXML | Submission_2025-11-10_07:00:14.045.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Sumio Ohtsuki |
| SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
| ModificationList | S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; O-phospho-L-serine; O-phospho-L-threonine |
| Instrument | instrument |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2024-11-10 19:06:23 | ID requested | |
| ⏵ 1 | 2025-11-10 07:00:14 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: mouse, podocyte, Cdkal1, DIA |
Contact List
| Sumio Ohtsuki | |
|---|---|
| lab head | |
| Sumio Ohtsuki | |
| contact affiliation | Kumamoto University |
| dataset submitter | |
Full Dataset Link List
| jPOST dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST003458/ |
