PXD057601-1

PXD057601 is an original dataset announced via ProteomeXchange.

Title	Cryopreservation of human spermatozoa using penetrating cryoprotectant-free vitrification medium exhibits altered proteomic profile compared to conventional rapid freezing method
Description	Vitrification is not considered as ideal method for cryopreservation of human spermatozoa due to the presence of high concentrations of penetrating cryoprotectants in the vitrification medium. Several studies in the literature suggest that the outcome of semen cryopreservation is superior when cryopreserved using conventional rapid freezing protocol compared to vitrification. The present study was aimed at assessing the functional and proteomic changes in the human spermatozoa cryopreserved in vitrification medium without penetrating cryoprotectant. Leftover ejaculates (n = 71) from men visiting the andrology laboratory for routine semen analysis were used. The liquefied semen samples were cryopreserved using rapid freezing and vitrification for a minimum of seven days. Post-thaw motility, mitochondrial function, DNA integrity, and acrosome intactness of spermatozoa were similar between the cryoprotectant-free vitrification and rapid freezing methods. The head morphology of spermatozoa cryopreserved by the vitrification method was better preserved compared to rapid freezing method. Proteomic analysis led to the identification of a total of 4378 proteins in spermatozoa. However, differential proteomic profile observed in spermatozoa cryopreserved with vitrification and conventional rapid freezing methods compared to spermatozoa from the ejaculate indicates the possible differences in the functional competence of the spermatozoa cryopreserved using these two protocols. Even though vitrification of spermatozoa in medium without penetrating cryoprotectant holds promise as an attractive option owing to the simplicity and rapidity of the protocol, further studies are necessary to understand the safety of the protocol and pregnancy outcome upon using the vitrified-thawed spermatozoa for assisted conception and assisted reproductive technology.
HostingRepository	PRIDE
AnnounceDate	2025-07-22
AnnouncementXML	Submission_2025-07-21_23:00:12.690.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Keshava Prasad T. S.
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-07 00:22:47	ID requested
⏵ 1	2025-07-21 23:00:13	announced

Dataset with its publication pending

submitter keyword: Conventional rapid freezing

Mitochondria

Motility

Acrosome

DNA damage

Ultrastructural changes

Dr. T. S. Keshava Prasad
contact affiliation	Professor and Deputy Director, Center for Systems Biology and Molecular Medicine, Yenepoya Research Centre, Yenepoya (Deemed to be University), Deralakatte, Mangalore
contact email	tskprasad@gmail.com
lab head
Keshava Prasad T. S.
contact affiliation	Professor and Deputy Director, Center for Systems Biology and Molecular Medicine, Yenepoya Research Centre, Yenepoya (Deemed to be University), Mangalore, India
contact email	keshav@ibioinformatics.org
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD057601
     1. Label: PRIDE project
     2. Name: Cryopreservation of human spermatozoa using penetrating cryoprotectant-free vitrification medium exhibits altered proteomic profile compared to conventional rapid freezing method