PXD057588-1

PXD057588 is an original dataset announced via ProteomeXchange.

Title	Quantitative cell type specific immunopeptidome analysis during macrophage and tumor co-evolution reveals therapeutic MHC-I restricted peptides in glioblastoma
Description	Glioblastoma treatment by immunotherapy is limited by the immunosuppresive tumor microenvironment, in part created by GBM associated macrophages. To uncover targetable MHC-I peptides for targeted immunotherapy, we performed cell type specific immunopeptidome analysis on primary macrophages and GBM tumor cells in a co-culture system to profile antigen presentation at the tumor:macrophage interface. Profiling the MHC-I restricted peptides revealed systematic antigen presentation alteration in both GBM tumors and macrophages during their co-evolution. We selected co-culture induced significantly altered peptides (CIVS peptides) which can serve as potential immunotherapy targets, and developed an mRNA vaccine encoding six selected CIVS peptides from GAMs and GBM tumor cells. Two doses of mRNA vaccination generated antigen specific immune response, significantly delayed GBM tumor growth, and in some cases eradicated tumor, demonstrating the translational potential of CIVS peptides as therapeutic targets for GBM/GAM targeting vaccines.
HostingRepository	PRIDE
AnnounceDate	2025-09-03
AnnouncementXML	Submission_2025-09-03_15:59:42.967.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD057588
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Yufei Cui
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-06 14:15:00	ID requested
⏵ 1	2025-09-03 15:59:43	announced

10.6019/PXD057588;

submitter keyword: tumor associated macrophage,MHC-I restricted peptides, Glioblastoma, immunopeptidome

Forest M.
contact affiliation	Department of Biological Engineering, Massachusetts Institute of Technology
contact email	fwhite@mit.edu
lab head
Yufei Cui
contact affiliation	MIT
contact email	yufeicui@mit.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD057588
     1. Label: PRIDE project
     2. Name: Quantitative cell type specific immunopeptidome analysis during macrophage and tumor co-evolution reveals therapeutic MHC-I restricted peptides in glioblastoma