PXD057585-1

PXD057585 is an original dataset announced via ProteomeXchange.

Title	High-Resolution Proteomics Unveils Salivary Gland Disruption and Saliva-Hemolymph Protein Exchange in Plasmodium-Infected Mosquitoes
Description	Plasmodium sporozoites, the stage that initiates a malaria infection, must invade the mosquito salivary glands before transmitting to a vertebrate host. However, the effects of sporozoite invasion on salivary gland physiology and saliva composition remain largely unexplored. We examined the impact of Plasmodium infection on Anopheles gambiae salivary glands using high-resolution proteomics, gene expression, and morphological analysis. The data revealed differential expression of various proteins, including the enrichment of humoral proteins in infected salivary glands originating from the hemolymph. These proteins diffused into the SGs due to structural damage caused by the sporozoites during invasion. Conversely, saliva proteins diffused out into the circulation of infected mosquitoes. Moreover, infection altered saliva protein composition, as shown by proteomes from saliva collected from mosquitoes infected by P. berghei or P. falciparum, revealing a significant reduction of immune proteins compared to uninfected mosquitoes. This reduction is likely due to the association of these proteins with the surface of sporozoites within the mosquito salivary secretory cavities. The saliva protein profiles from mosquitoes infected with both Plasmodium species were remarkably similar, suggesting a conserved interaction between sporozoites and salivary glands. Our results provide a foundation for understanding the molecular interactions between Plasmodium sporozoites and mosquito salivary glands.
HostingRepository	PRIDE
AnnounceDate	2025-12-01
AnnouncementXML	Submission_2025-11-30_16:49:05.207.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thiago Silva
SpeciesList	scientific name: Plasmodium berghei ANKA; NCBI TaxID: NEWT:5823; scientific name: Anopheles gambiae str. PEST; NCBI TaxID: NEWT:180454;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-06 12:47:35	ID requested
⏵ 1	2025-11-30 16:49:06	announced

10.1038/s41467-025-64837-6;

Alves E Silva TL, Kanatani S, Barletta Ferreira AB, Schwartz CL, Liffner B, Talyuli OAC, Olivas J, Nagata BM, Pala ZR, Pascini T, Alves DA, Zhao M, Suzuki M, Dorner LP, Frischknecht F, Coppens I, Barillas-Mury C, Absalon S, Ribeiro JMC, Sinnis P, Vega-Rodriguez J, High-resolution proteomics unveils salivary gland disruption and saliva-hemolymph protein exchange in Plasmodium-infected mosquitoes. Nat Commun, 16(1):10205(2025) [pubmed]

submitter keyword: Infection,Mosquito, Saliva, Proteome, Salivary gland, Plasmodium

Joel Vega Rodriguez
contact affiliation	Molecular Parasitology and Entomology Unit,Laboratory of Malaria and Vector Research, National Institutes of Health. USA.
contact email	joel.vega-rodriguez@nih.gov
lab head
Thiago Silva
contact affiliation	National Institutes of Health
contact email	thiagoluiz.alvesesilva@nih.gov
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/12/PXD057585

PRIDE project URI

• PRIDE
  1. PXD057585
     1. Label: PRIDE project
     2. Name: High-Resolution Proteomics Unveils Salivary Gland Disruption and Saliva-Hemolymph Protein Exchange in Plasmodium-Infected Mosquitoes