PXD057584-1

PXD057584 is an original dataset announced via ProteomeXchange.

Title	Surface Protein Hexosylation in Natural and Synthetic Minimal Genome Bacteria
Description	This study describes surface protein hexosylation in Mycoplasma genitalium, a human urogenital pathogen with a 580-kbp genome; the ruminant pathogen Mycoplasma mycoides subsp. capri; and JCVI-syn3A, a bacterium with a synthetic minimal M. mycoides 543-kbp gene set for growth under axenic conditions. Previous work from our group described a surface protein glycosylation system in the murine pathogens M. pulmonis and M. arthritidis that utilizes hexoses cleaved from oligo-/polysaccharides as glycosyl donors and does not utilize a consensus sequence for glycosyl acceptors. As we have been unable to find a glycosylation knockout while screening mycoplasmas, we hypothesized the hexosylation system was active in these minimal genome mycoplasmas as well as JCVI-Syn3A's M. mycodies parent organism. Our data indicates hexosylation is maintaned in these organisms, and remarkably, the available pool of glycosyl acceptors includes aspartic acid and glutamic acid.
HostingRepository	PRIDE
AnnounceDate	2025-08-01
AnnouncementXML	Submission_2025-08-01_08:46:08.064.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD057584
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	John Sanford
SpeciesList	scientific name: Mycoplasma mycoides subsp. capri str. GM12; NCBI TaxID: 436113; scientific name: Mycoplasmoides genitalium G37; NCBI TaxID: 243273; scientific name: synthetic bacterium JCVI-Syn3A; NCBI TaxID: 2144189;
ModificationList	acetylated residue; monohydroxylated residue; hexosylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos Pro

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-06 12:33:36	ID requested
⏵ 1	2025-08-01 08:46:08	announced

10.1371/JOURNAL.PONE.0329506;

10.6019/PXD057584;

submitter keyword: Glycosylation, Mycoplasma mycoides, Mycoplasmoides genitalium,Mycoplasma genitalium, JCVI-Syn3A, Hexosylation

Thomas Prescott
contact affiliation	Department of Pediatrics, University of Alabama at Birmingham, United States of America.
contact email	tatkinso@uabmc.edu
lab head
John Sanford
contact affiliation	University of Alabama at Bimringham
contact email	jsanfor4@uab.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD057584
     1. Label: PRIDE project
     2. Name: Surface Protein Hexosylation in Natural and Synthetic Minimal Genome Bacteria