PXD057583-1

PXD057583 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Mass Spectrometry-Based Proteomics of Extracellular Vesicle Proteins (EVPs)
Description	For proteomic analysis, isolated EVPs were subjected to mass spectrometry (MS). Briefly, proteins were extracted from EVP pellets, and concentrations were measured using the Pierce MicroBCA kit. Each sample underwent processing with 20 micrograms of protein, first reduced with DTT, then alkylated with iodoacetamide, and finally digested with trypsin in 25 mM NH4HCO3. The resulting tryptic peptides were cleaned using a C18 column and reconstituted in 25 microliters of 0.1% formic acid; 12 microliters of this solution was analyzed by LC-MS/MS over a 110-minute run.
HostingRepository	MassIVE
AnnounceDate	2024-11-06
AnnouncementXML	Submission_2024-11-06_11:29:57.603.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Nathan
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	instrument model

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-06 11:20:23	ID requested
⏵ 1	2024-11-06 11:29:58	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: trophoblast stem cell secretome, extracellular vesicles and particles, exosomes, senescence, aging, DatasetType:Proteomics

submitter keyword: trophoblast stem cell secretome, extracellular vesicles and particles, exosomes, senescence, aging, DatasetType:Proteomics

Contact List

Kotb Abdelmohsen
contact affiliation	Staff Scientist
contact email	abdelmohsenk@mail.nih.gov
lab head
Nathan
contact affiliation	Temple University
contact email	tun37257@temple.edu
dataset submitter

Full Dataset Link List

MassIVE dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v07/MSV000096320/

MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v07/MSV000096320/