PXD057575 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Delayed protein translocation protects mitochondria against toxic CAT-tailed proteins |
| Description | In the ribosome-associated quality control acting on nuclear-encoded mitochondrial proteins (mitoRQC), Vms1 protects mitochondria from toxic effects of Rqc2-generated C-terminal alanyl and threonyl (CAT) tailed proteins that escaped proteosomal degradation facilitated by E3 ubiquitin ligase Ltn1. Here, we performed a genome-wide screen in yeast to identify novel proteins involved in mitoRQC. We found that Pth2, a peptidyl-tRNA hydrolase in the outer mitochondrial membrane, influences aggregation of mitochondrial-targeted CAT-tailed proteins without majorly affecting the CAT-tailing process itself. Peptidyl-tRNA hydrolase activity is essential during this process, however, the activity of Pth2 can be substituted by another peptidyl-tRNA hydrolase, upon proper localization. Our data suggest that Pth2 acts through modulating protein import into mitochondria, enabling CAT-tailed proteins to get access to the cytosolic chaperones and thus relieving the mitochondrial proteostasis network. Other hits obtained in the screen show that, in general, slowing down protein translocation protects mitochondria against toxic CAT-tailed proteins. |
| HostingRepository | PRIDE |
| AnnounceDate | 2025-11-13 |
| AnnouncementXML | Submission_2025-11-13_08:44:14.715.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Serena Schwenkert |
| SpeciesList | scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: NEWT:4932; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | impact II |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2024-11-06 05:33:35 | ID requested | |
| ⏵ 1 | 2025-11-13 08:44:15 | announced | |
Publication List
| 10.1083/jcb.202411107; |
| Schreiner A, Heim A, Pletschacher L, Alznauer LM, Schwenkert S, Wolff F, Zanin E, Mikeladze-Dvali T, PCMD-1 stabilizes the PCM scaffold and facilitates centriole separation. J Cell Biol, 224(12):(2025) [pubmed] |
Keyword List
| submitter keyword: Yeast, quality control, mitochondria |
Contact List
| Serena Schwenkert |
|---|
| contact affiliation | Faculty of Biology, MSBioLMU, LMU München, Großhaderner Str. 2-4, 82152, Planegg, Germany |
| contact email | serena.schwenkert@lmu.de |
| lab head | |
| Serena Schwenkert |
|---|
| contact affiliation | LMU Munich |
| contact email | serena.schwenkert@lmu.de |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD057575
- Label: PRIDE project
- Name: Delayed protein translocation protects mitochondria against toxic CAT-tailed proteins
