PXD057558-2

PXD057558 is an original dataset announced via ProteomeXchange.

Title	Glioblastoma-derived migrasomes promote migration and invasion by releasing PAK4 and LAMA4
Description	Almost all high-grade gliomas, particularly glioblastoma (GBM), are highly migratory and aggressive. Migrasomes are organelles produced by highly migratory cells capable of mediating intercellular communication. Thus, GBM cells may produce migrasomes during migration. However, it remains unclear whether migrasomes can influence GBM migration and invasion. In this study, we observed the presence and formation of migrasomes in GBM cells. We found that expression levels of key migrasome formation factor, tetraspanin 4 (TSPAN4), correlated positively with pathological grade and poor prognosis of GBM based on the databases and clinical samples analysis. Subsequently, we knocked down TSPAN4 and found that GBM migration and invasion were significantly inhibited due to the reduced formation of migrasomes. We further confirmed that migrasomes are enriched in extracellular matrix (ECM)-related proteins such as p21-activating kinase 4 (PAK4) and laminin alpha 4 (LAMA4). Our experimental results suggest that migrasomes promote GBM migration by releasing such proteins into the extracellular space. Overall, we identified migrasomes in GBM and the molecular mechanisms by which they regulate them, providing potential targets for treating GBM.
HostingRepository	iProX
AnnounceDate	2024-11-05
AnnouncementXML	Submission_2025-08-20_19:29:51.741.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zhe Huang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion ETD

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-05 18:55:34	ID requested
1	2025-08-20 19:29:15	announced
⏵ 2	2025-08-20 19:29:52	announced	2025-08-21: Add info.

Huang Z, Wang M, Chen Y, Tang H, Tang K, Zhao M, Yang W, Zhou Z, Tian J, Xiang W, Li S, Luo Q, Liu L, Zhao Y, Li T, Zhou J, Chen L, Glioblastoma-derived migrasomes promote migration and invasion by releasing PAK4 and LAMA4. Commun Biol, 8(1):91(2025) [pubmed]

submitter keyword: Q-MS, Migrasome, Cell Body

Ligang Chen
contact affiliation	Department of Neurosurgery, The Affiliated Hospital, Southwest Medical University
contact email	chengligang.cool@163.com
lab head
Zhe Huang
contact affiliation	Department of Neurosurgery, The Affiliated Hospital, Southwest Medical University
contact email	huangzhe9810@163.com
dataset submitter

iProX dataset URI