PXD057540-1

PXD057540 is an original dataset announced via ProteomeXchange.

Title	Mouse hepatocyte conditioned media LC-MS/MS.
Description	Hepatocyte caspase-8 is elevated in MASH and promotes fibrosis independently of apoptosis changes. To investigate how hepatocyte caspase-8 might drive liver fibrosis progression in MASH, we focused on activated hepatic stellate cells (HSCs), which are the primary source of collagen-producing myofibroblasts and central players in MASH fibrosis. To assess a potential link between hepatocyte caspase-8 and HSC activation, we used an ex vivo model where primary murine HSCs were cultured with conditioned media from siCasp8-treated or control primary hepatocytes. Supporting our hypothesis, HSC activation markers were reduced in HSCs exposed to conditioned media from Casp8-silenced AML12 hepatocytes compared to controls. To pinpoint caspase-8-dependent secretory proteins that could activate HSCs, we conducted LC-MS/MS on conditioned media from these cells, identifying secretory proteins reduced in Casp8-silenced AML12 hepatocytes.
HostingRepository	PRIDE
AnnounceDate	2025-07-09
AnnouncementXML	Submission_2025-07-09_07:00:08.493.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rajesh Soni
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-05 07:40:06	ID requested
⏵ 1	2025-07-09 07:00:08	announced

Dataset with its publication pending

submitter keyword: AML12, hepatocyte, conditioned media, LC-MS/MS

Ira Tabas
contact affiliation	Professor of Anatomy & Cell Biology (in Physiology and Cellular Biophysics) Columbia University Irving Medical Center
contact email	iat1@columbia.edu
lab head
Rajesh Soni
contact affiliation	Columbia University Medical center
contact email	rs3869@cumc.columbia.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/07/PXD057540

PRIDE project URI

• PRIDE
  1. PXD057540
     1. Label: PRIDE project
     2. Name: Mouse hepatocyte conditioned media LC-MS/MS.