PXD057493-1

PXD057493 is an original dataset announced via ProteomeXchange.

Title	Discovery of tomato UDP-glucosyltransferases involved in bioactive jasmonate homeostasis using limited proteolysis-coupled mass spectrometry
Description	Jasmonic acid (JA) is the precursor of the plant hormone, jasmonoyl-isoleucine, with roles in defences and development. Molecular signalling pathways of JA are well-understood, but it is still not fully understood how JA is metabolised. We used limited proteolysis-coupled mass spectrometry to search for jasmonic acid (JA) interacting proteins in tomato with the aim of finding missing components of JA metabolism. We identified several UDP-glycosyltransferases (UGTs) that might be involved in direct glycosylation of JA, a metabolic step that is catalyzed by yet unknown enzymes. Two of the candidates from our dataset were confirmed to glycosylate JA in vitro to create JA-glucosyl ester (JA-Glc). JA-Glc was shown to have less biological activity than JA, suggesting that the discovered UGTs may be involved in inactivating JA for storage or catabolism.
HostingRepository	PRIDE
AnnounceDate	2026-08-27
AnnouncementXML	Submission_2026-08-27_08:53:25.136.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kresimir Sola
SpeciesList	scientific name: Solanum lycopersicum; NCBI TaxID: NEWT:4081;
ModificationList	monohydroxylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-04 09:30:34	ID requested
⏵ 1	2026-08-27 08:53:25	announced

Dataset with its publication pending

submitter keyword: jasmonic acid, LiP-MS, protein-metabolite interactome,Tomato

Alain Goossens
contact affiliation	Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium VIB Center for Plant Systems Biology, B-9052 Ghent, Belgium
contact email	alain.goossens@psb.vib-ugent.be
lab head
Kresimir Sola
contact affiliation	VIB-UGent Center for Plant Systems Biology, Technologiepark-Zwijnaarde 71, 9052 Ghent, Belgium
contact email	kresimir.sola@psb.vib-ugent.be
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD057493
     1. Label: PRIDE project
     2. Name: Discovery of tomato UDP-glucosyltransferases involved in bioactive jasmonate homeostasis using limited proteolysis-coupled mass spectrometry