PXD057460-1

PXD057460 is an original dataset announced via ProteomeXchange.

Title	Large Extracellular Vesicles Derived from LPS-preconditioned Cardiomyocytes Alleviate Myocarditis via Mediating Macrophage Polarization and Modulating p38 MAPK Pathway
Description	Myocarditis is an inflammatory injury to the myocardium characterized by disrupted intercellular communication, involving macrophages and cardiomyocytes as key players. However, the interactions between macrophages and cardiomyocytes during myocarditis remain inadequately explored. Emerging evidence indicated that extracellular vesicles (EVs) play a crucial role in intercellular communication. Here, we demonstrated that large EVs derived from lipopolysaccharide (LPS)-preconditioned cardiomyocytes (C-lEVLPS) exhibited anti-inflammatory effects on macrophages and alleviated cardiac inflammation and dysfunction in a mouse model of CVB3-induced myocarditis. Additionally, C-lEVLPS facilitated macrophage polarization toward the M2-like phenotype and inhibits M1 polarization, both in vitro and in vivo. label-free proteomics analysis showed, compared to phosphate-buffered saline (PBS)-preconditioned cardiomyocytes (C-lEVPBS), C-lEVLPS was enriched in the phosphatase 2 scaffold subunit alpha protein (PP2AA), which can recruit other subunits to form the PP2A complex, ultimately leading to the dephosphorylates of p38. This study highlights the effect of C-lEVLPS in myocarditis and uncovers the potential mechanism that modulates macrophage polarization by delivering PP2AA from cardiomyocytes to macrophages and regulating the p38 MAPK pathway. These findings provide a promising therapeutic strategy for myocarditis.
HostingRepository	PRIDE
AnnounceDate	2025-09-29
AnnouncementXML	Submission_2025-09-28_16:11:21.304.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yingnan You
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro 2

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-03 00:33:20	ID requested
⏵ 1	2025-09-28 16:11:22	announced

Jiang Y, You Y, Xie Y, Zhou S, Ma M, Han B, Large extracellular vesicles derived from LPS-preconditioned cardiomyocytes alleviate myocarditis via mediating macrophage polarization and modulating p38 MAPK pathway. Front Immunol, 16():1629676(2025) [pubmed]

10.3389/fimmu.2025.1629676;

ProteomeXchange project tag: Biology/Disease-Driven Human Proteome Project (B/D-HPP), Human Proteome Project

submitter keyword: myocarditis;cardiomyocytes

macrophages

large extracellular vesicles

Yanjie Jiang
contact affiliation	Shandong Provincial Hospital
contact email	15650095567@163.com
lab head
Yingnan You
contact affiliation	Shandong provincial hospital
contact email	13998302426@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/09/PXD057460

PRIDE project URI

• PRIDE
  1. PXD057460
     1. Label: PRIDE project
     2. Name: Large Extracellular Vesicles Derived from LPS-preconditioned Cardiomyocytes Alleviate Myocarditis via Mediating Macrophage Polarization and Modulating p38 MAPK Pathway