PXD057405-1

PXD057405 is an original dataset announced via ProteomeXchange.

Title	Trim32-DPEP2 axis is an inflammatory switch of macrophages in intestinal inflammation
Description	The mechanisms that mediate inflammatory macrophages in intestinal inflammation are incompletely understood. Here, using merged analysis of RNA sequences and mass spectrometry (MS)-based quantitative proteomics, we report the distinction of proteomics and transcriptomics in activated macrophage. Dipeptidase-2 (DPEP2), belonging to DPEP family, is highly expressed and downregulated sharply at protein level but not mRNA level in macrophages responding to inflammatory stimulations. Repression of DPEP2 not only enhances macrophage-mediated intestinal inflammation in vivo but also promotes the production of ROS and the transduction of inflammatory pathways in macrophages in vitro. Mechanistically, DPEP2 inhibits recruitment of inactivated macrophages to inflammatory sites by impairing activation of vimentin (VIM), and DPEP2 downregulation promotes the infiltration of pro-inflammatory macrophages through enhancing the activation of VIM by Akt1. Besides, overexpressed DPEP2 inhibits the transduction of inflammatory signals by resisting MAK3K7 in inactivated macrophages, and DPEP2 is degraded by activated Trim32 resulting in sharp activation of NF-κB and p38 MAPK signals by released MAK3K7 in pro-inflammatory macrophages during the development of intestinal inflammation. Trim32-DPEP2 axis accumulates potential energy of inflammation for macrophages. These results identify DPEP2 as a key regulator of macrophage-mediated intestinal inflammation. Thus, Trim32-DPEP2 axis may be a potential therapeutic target for intestinal inflammation.
HostingRepository	PRIDE
AnnounceDate	2025-07-28
AnnouncementXML	Submission_2025-07-27_16:06:24.178.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zhiyan Zhan
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Thermo Fisher Scientific instrument model

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-31 09:27:44	ID requested
⏵ 1	2025-07-27 16:06:25	announced

10.1038/s41418-025-01468-w;

Zhan Z, Liang H, Zhao Z, Pan L, Li J, Chen Y, Xie Z, Yan Z, Xiang Y, Liu W, Hong L, The Trim32-DPEP2 axis is an inflammatory switch in macrophages during intestinal inflammation. Cell Death Differ, 32(7):1336-1352(2025) [pubmed]

submitter keyword: intestinal inflammation

DPEP2

macrophage

VIM

MAP3K7

Trim32

Zhiyan Zhan
contact affiliation	1 Department of Clinical Nutrition, Shanghai Children's Medical Center, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200127, China 2 Clinical Research Center, Shanghai Children's Medical Center, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200127, China
contact email	zhanzhiyan@sjtu.edu.cn
lab head
Zhiyan Zhan
contact affiliation	shanghai jiao tong university
contact email	zhanzhiyan@sjtu.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD057405
     1. Label: PRIDE project
     2. Name: Trim32-DPEP2 axis is an inflammatory switch of macrophages in intestinal inflammation