PXD057344-1

PXD057344 is an original dataset announced via ProteomeXchange.

Title	Thiol metabolic heterogeneity in immune cells causes distinct molecular mechanisms of toxicity upon chronic lead exposure
Description	Lead pollution is global, billions of people are chronically exposed to lead. Biological thiols are nature sequesters of lead. Human immune system is highly vulnerable to lead, unfortunately the underlying mechanism remains unknown. Using mass cytometry and mass spectrometry, we systematically identified lead targets in immune cells from mice model of chronic lead exposure. We found CD4+ T and neutrophil are most vulnerable to lead, respectively due to low thiol and high glutathione metabolism. Specifically, in CD4+ T, lack of thiol renders severe damage of ribosome by lead, which we successfully rescued using thiol drugs. In neutrophil, robust glutathione metabolism accumulates lead, which can be passed on to proteins of higher binding affinity. Among these proteins, our evidences suggest lead suppresses immune activation through PKC-like, PE/DAG-binding domain on RAF1 and PKCs. Overall, it clarified the mechanisms of toxicity in immune cells upon chronic lead exposure, which provides valuable information for medical care and public health.
HostingRepository	iProX
AnnounceDate	2024-10-30
AnnouncementXML	Submission_2024-10-30_00:42:52.914.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yifan Hong
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090; scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF-X; Bruker Daltonics amaZon series

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-30 00:39:03	ID requested
⏵ 1	2024-10-30 00:42:53	announced

Dataset with its publication pending

submitter keyword: chronic lead exposure, thiol metabolism, immunotoxicity, CD4+ T, neutrophil

Yifan Hong
contact affiliation	Shenzhen Bay Laboratory
contact email	angivan@163.com
lab head
Yifan Hong
contact affiliation	Shenzhen Bay Laboratory
contact email	angivan@163.com
dataset submitter

iProX dataset URI