PXD057264-1

PXD057264 is an original dataset announced via ProteomeXchange.

Title	Unexpected role of a truncated ribosome-associated chloroplast trigger factor homolog serving ribosome biogenesis in plants - Data set 2: Analysis of sedimented ribosomes
Description	Molecular chaperones are essential throughout a protein's life and act already during protein synthesis. Bacteria and chloroplasts of plant cells share the ribosome-associated chaperone trigger factor (Tig1 in plastids), facilitating maturation of emerging nascent polypeptides. While typical trigger factor chaperones employ three domains for their task, we identified a truncated form, Tig2, containing just the ribosome binding domain. Tig2 is predominantly found within higher-evolved photosynthetic species and appears to have acquired an entirely different tasks than co-translational folding. Tig2 deletion results in remarkable leaf developmental defects of cold-exposed Arabidopsis thaliana plants. Our data indicate that Tig2 functions during ribosome assembly, specifically promoting the hidden break formation within the 23S rRNA, the final step of chloroplast ribosome biogenesis. We hypothesize that Tig2 binding to the tunnel-exit surface serves protecting this sensitive surface during ribosome assembly, illustrating a fascinating concept of how similar chaperone domains have evolved for different molecular tasks.
HostingRepository	PRIDE
AnnounceDate	2024-12-09
AnnouncementXML	Submission_2024-12-09_07:58:38.880.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Markus Räschle
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-28 09:44:27	ID requested
⏵ 1	2024-12-09 07:58:39	announced

Dataset with its publication pending

submitter keyword: Ribosomes, Chaperones,Arabidopsis

Markus Räschle
contact affiliation	Molecular Genetics, RPTU Kaiserslautern
contact email	raeschle@rhrk.uni-kl.de
lab head
Markus Räschle
contact affiliation	Technical University Kaiserslautern
contact email	raeschle@rhrk.uni-kl.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD057264
     1. Label: PRIDE project
     2. Name: Unexpected role of a truncated ribosome-associated chloroplast trigger factor homolog serving ribosome biogenesis in plants - Data set 2: Analysis of sedimented ribosomes