PXD057248-1

PXD057248 is an original dataset announced via ProteomeXchange.

Title	Increase in harmful R-loops associates with different alterations in mRNP composition
Description	Understanding how the assembly of nascent mRNA into a ribonucleoprotein (mRNP) influences R-loop homeostasis is crucial for gaining insights into the cellular mechanisms that prevent genome instability. Here, we identify three RNA-binding proteins, Rie1, Rim4 and She2, whose stoichiometry is important for limiting R-loop accumulation and, consequently, preventing DNA damage. Notably, Rim4 and She2 are overrepresented in mRNPs assembled in the absence of THO. Additionally, we found that an excess of Dis3 impairs exosome function, promoting R-loops, particularly from non-coding RNAs, that causes genomic instability. Our results indicate that mRNP assembly is a precise process that when perturbed by changes in the availability of different RBPs or RNAs, causes R-loop-mediated DNA damage in the cell. These results may have important implications for understanding the mechanism that promotes cancer, as several RBPs are overexpressed in different types of tumors.
HostingRepository	PRIDE
AnnounceDate	2025-06-13
AnnouncementXML	Submission_2025-06-13_08:30:39.688.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Guillaume CHEVREUX
SpeciesList	scientific name: Saccharomyces cerevisiae; NCBI TaxID: NCBITaxon:4932; scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue; deamidated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-28 03:45:28	ID requested
⏵ 1	2025-06-13 08:30:40	announced

Dataset with its publication pending

submitter keyword: R-loops, THO complex, RNA-binding proteins,messenger ribonucleoparticle

Ana G. Rondón
contact affiliation	Centro Andaluz de Biología Molecular y Medicina Regenerativa (CABIMER), Universidad de Sevilla-CSIC-Universidad Pablo de Olavide, Seville, Spain Departamento de Genética, Facultad de Biología, Universidad de Sevilla, Seville, Spain
contact email	ana.rondon@cabimer.es
lab head
Guillaume CHEVREUX
contact affiliation	Institut Jacques Monod, CNRS UMR7592
contact email	guillaume.chevreux@ijm.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/06/PXD057248

PRIDE project URI

• PRIDE
  1. PXD057248
     1. Label: PRIDE project
     2. Name: Increase in harmful R-loops associates with different alterations in mRNP composition