PXD057244-1

PXD057244 is an original dataset announced via ProteomeXchange.

Title	The lipid transport Mla Pqi Chimeric system is essential for Brucella abortus survival in macrophages
Description	Envelope integrity is essential for diderm bacteria. The envelope is composed of an inner membrane (IM) and an outer membrane (OM), which are separated by a periplasmic space. Several pathways have been recently identified that facilitate the transport of phospholipids between the two membranes in Escherichia coli, including the maintenance of OM lipid asymmetry (Mla) and paraquat inducible (Pqi) systems. In this study, we report the identification and characterisation of a complex named Mpc in the intracellular pathogen Brucella abortus. Mpc is conserved in numerous species of Hyphomicrobiales and exhibits homology to both the Mla and Pqi systems. Mpc is essential for bacterial growth under conditions of envelope stress and for survival within macrophages during the early stages of infection. Analyses of protein-protein interactions and structural predictions indicate that the Mpc complex forms a stable entity that spans the entire periplasmic space. The absence of this system results in an altered lipid composition of the OM vesicles, which supports the hypothesis that it plays a role in the trafficking of lipids between the IM and OM. The discovery of a novel lipid trafficking system enhances the diversity and complexity of known lipid trafficking systems within diderm bacteria.
HostingRepository	PRIDE
AnnounceDate	2025-06-19
AnnouncementXML	Submission_2025-06-19_05:15:48.942.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD057244
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Marc Dieu
SpeciesList	scientific name: Brucella abortus; NCBI TaxID: 235;
ModificationList	acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-28 02:34:01	ID requested
⏵ 1	2025-06-19 05:15:49	announced

10.6019/PXD057244;

submitter keyword: Lipid Transport

Outer Membrane Biogenesis

Gram Negative Cell Envelope

Pqi

Mla

Envelope Spanning

Brucella

Xavier De Bolle
contact affiliation	Research Unit in Biology of Microorganisms (URBM), Namur Research Institute for life Sciences (Narilis), University of Namur, Namur, Belgium
contact email	xavier.debolle@unamur.be
lab head
Marc Dieu
contact affiliation	University of Namur - MaSUN
contact email	marc.dieu@unamur.be
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD057244
     1. Label: PRIDE project
     2. Name: The lipid transport Mla Pqi Chimeric system is essential for Brucella abortus survival in macrophages