PXD057221-1

PXD057221 is an original dataset announced via ProteomeXchange.

Title	Proteome profiling of Cucurbita pepo phyllosphere after infection by Podosphaera xanthii and application of Reynoutria sachalinensis extract
Description	Podosphaera xanthii is the main causal agent of powdery mildew (PM) disease for Cucurbita pepo. Disease control is attained principally by applications of chemical fungicides, along with parallel use of tolerant crop varieties and alternate application of elicitors to control development of disease resistance. To get insight into C. pepo molecular responses to P. xanthii infection and elicitor treatment we studied the proteomic profile differences at the phyllosphere of a zucchini cultivar susceptible to PM, at the onset of P. xanthii (PX) infection and after application of Reynoutria sachalinensis (RS) plant extract, respectively, using a nano-LC-HRMS/MS, Q-Exactive-Orbitrap approach. Analysis of peptide sequences regarding four treatment groups (Control; PX; RS; and RSPX (PX-infected priorly treated with RS)) resulted in 2070 CuGenDB annotations. Three comparisons (treatments vs Control) encompassed most of the Differentially Expressed Proteins (DEPs). In these three comparisons, KEGG and Gene Ontology functional analyses highlighted unique differentially enriched pathways -some of which including highly expressed proteins- in PX-related (Proteasome, Pentose phosphate pathway, and Carbon fixation), RS-related (Biosynthesis of Secondary metabolites, Flavonoids, and Starch and Sucrose metabolism), and RSPX-related (Pyruvate metabolism and Polycomb repressive complex) comparisons respectively, suggesting distinct mechanisms of early plant responses modulated by PX and RS. Furthermore, in four out of six comparisons the Thiamine metabolism pathway was found to be enriched, suggesting a pivotal role in PX-induced responses.
HostingRepository	PRIDE
AnnounceDate	2024-11-07
AnnouncementXML	Submission_2024-11-07_14:03:33.342.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD057221
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Ioannis Theologidis
SpeciesList	scientific name: Cucurbita pepo; NCBI TaxID: 3663;
ModificationList	acetylated residue; monohydroxylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-26 14:30:02	ID requested
⏵ 1	2024-11-07 14:03:33	announced

10.6019/PXD057221;

10.3390/APP142110061;

submitter keyword: Cucurbita pepo

zucchini

Podosphaera xanthii

powdery mildew

Reynoutria sachalinensis

elicitors

proteomics

nano-LC-HRMS/MS

Q-Exactive-Orbitrap

Dimosthenis Kizis
contact affiliation	Laboratory of Mycology, Scientific Directorate of Phytopathology, Benaki Phytopathological Institute, 8 St. Delta Street, 14561, Kifissia, Attica, Greece
contact email	d.kizis@bpi.gr
lab head
Ioannis Theologidis
contact affiliation	Laboratory of Toxicological Control of Pesticides, Scientific Directorate of Pesticides’ Control & Phyto-pharmacy, Benaki Phytopathological Institute, 8 St. Delta Street, 14561, Kifissia
contact email	i.theologidis@bpi.gr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/11/PXD057221

PRIDE project URI

• PRIDE
  1. PXD057221
     1. Label: PRIDE project
     2. Name: Proteome profiling of Cucurbita pepo phyllosphere after infection by Podosphaera xanthii and application of Reynoutria sachalinensis extract