PXD057165-1

PXD057165 is an original dataset announced via ProteomeXchange.

Title	Laser microdissection and mass spectrometry shows loss of podocyte proteins in minimal change disease and focal segmental glomerulosclerosis.
Description	Minimal change disease and primary focal segmental glomerulosclerosis (FSGS) are common causes of nephrotic syndrome. Both diseases present with massive proteinuria and widespread foot process effacement on electron microscopy examination. On the other hand, secondary FSGS can also present with significant proteinuria, although the extent of foot process effacement can be variable. In this study, we performed laser microdissection of glomeruli followed by mass spectrometry to study podocyte proteins and compare the differences between the 3 diseases. Our study shows significant decrease in total spectral counts of nephrin, podocin, CD2-associated protein, alpha actinin 4, inverted formin 2 and dystroglycan 1 in all 3 diseases compared to time zero kidney transplant controls and membranous nephropathy biopsies (p <0.001). On the other hand, the decrease in the podocyte proteins in minimal change disease, primary FSGS and secondary FSGS was comparable and there was no significant difference. Surprisingly, there was no significant decrease in the podocyte proteins in membranous nephropathy compared to time zero kidney transplant control biopsies. In conclusion, our study shows significant decrease in major podocyte proteins in minimal change disease, primary and secondary FSGS, but not in time zero kidney transplant controls and in membranous nephropathy.
HostingRepository	PRIDE
AnnounceDate	2025-12-29
AnnouncementXML	Submission_2025-12-28_16:54:39.458.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD057165
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Benjamin Madden
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-24 15:48:26	ID requested
⏵ 1	2025-12-28 16:54:40	announced

10.6019/PXD057165;

10.1016/j.ekir.2025.09.043;

Fervenza FC, Vargas-Brochero MJ, Debiec H, Madden B, Andrade L, Zand L, Ronco P, Sethi S, Mass Spectrometry of Minimal Change Disease and Focal Segmental Glomerulosclerosis. Kidney Int Rep, 10(12):4219-4233(2025) [pubmed]

submitter keyword: laser microdissection, focal segmental glomerulosclerosis, mass spectrometry,minimal change disease

Sanjeev Sethi
contact affiliation	Professor of Laboratory Medicine and Pathology; ConsultantPathology
contact email	Sethi.Sanjeev@mayo.edu
lab head
Benjamin Madden
contact affiliation	Mayo Clinic
contact email	madden.benjamin@mayo.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD057165
     1. Label: PRIDE project
     2. Name: Laser microdissection and mass spectrometry shows loss of podocyte proteins in minimal change disease and focal segmental glomerulosclerosis.