PXD057162-1

PXD057162 is an original dataset announced via ProteomeXchange.

Title	Identification of cellular factors associated with HIV-1 virions isolated by filtration and ultracentrifugation. (Total lysate)
Description	The aim of this project was to identify cellular factors enriched with HIV-1 virions after filtration and ultracentrifugation of culture surpernatants of HIV-1 producer cells compared to a control. To this end, HeLa cells were transfected with HIV-1 NL4-3 provirus and, 48 hours after transfection, culture surpernatants of HIV-1 producer cells were collected, filtered and ultracentrifugated on a 20% sucrose cushion to pellet viruses released in the supernatants. As a control, supernatant from mock-transfected HeLa cells was collected and prepared as the supernatant of HIV-1 producer cells. The same volume of filtered and ultracentrifugated supernatants from mock- and HIV-1-producing cells were then subjected to LC-MS analysis
HostingRepository	PRIDE
AnnounceDate	2025-10-08
AnnouncementXML	Submission_2025-10-08_08:46:07.078.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marjorie Leduc
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-24 13:25:12	ID requested
⏵ 1	2025-10-08 08:46:07	announced

Dataset with its publication pending

submitter keyword: HIV-1, virion purification, ATG proteins, cellular factors

Clarisse Berlioz-Torrent
contact affiliation	Université Paris Cité, Institut Cochin, INSERM, CNRS, F-75014 PARIS, France
contact email	clarisse.berlioz@inserm.fr
lab head
Marjorie Leduc
contact affiliation	Plateforme protéomique 3P5, Université de Paris, Institut Cochin, INSERM, CNRS,
contact email	marjorie.leduc@inserm.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/10/PXD057162

PRIDE project URI

• PRIDE
  1. PXD057162
     1. Label: PRIDE project
     2. Name: Identification of cellular factors associated with HIV-1 virions isolated by filtration and ultracentrifugation. (Total lysate)