PXD057155-1

PXD057155 is an original dataset announced via ProteomeXchange.

Title	ALTERED PATTERN OF PROTEOLYSIS OF EXTRACELLULAR PROTEINS IN RHEGMATOGENOUS RETINAL DETACHMENT BY MINING OF TRYPTIC AND N-TERMINI DATASETS FROM VITREOUS HUMOR PROTEOME
Description	Retinal detachment is a severe eye condition characterized by the detachment of the neurosensory retina from the retinal pigment epithelium and caused by retinal tears. Pars plana vitrectomy is the elective surgical procedure during which vitreous humor, a fluid which shapes the eye globe and supports mechanically the adherence of the retina to the posterior chamber of the eye, is collected. Therefore, profiling of the vitreous humor proteome of subjects diagnosed with retinal detachment is supposed to provide molecular evidence on the pathobiology of the disease and on its secondary complications, such as proliferative vitreo-retinopathy, which predispose to recurrent RD (observed in 20% of cases), a sight threatening condition. Herein, we investigated the perturbations of vitreous proteome between subjects affected by primary retinal detachment and controls by shot-gun proteomics approaches. Proteomic datasets were first analyzed and searched for global proteome changes. Thereafter, starting from the assumption that the disease could be sustained by altered proteolytic processing of structural and non-structural elements of the VH, mining of N- and C-termini was performed to uncover endogenous proteolytic events underscoring the disease condition. The search retrieved evidence of a wide repertoire of either previously characterized or uncharacterized proteolytic events. Most notably, comparison of the N- and C-termini landscape between experimental groups highlighted robust alterations in the repertoire of cleaved proteins. Further strengthened by immunoblotting studies on a select panel of proteins, the data envisage that retinal detachment is promoted or characterized, as a secondary process, by proteolytic-based alterations of structural and non-structural components involved in the regulation of immune process, proteases inhibition and most notably, angiogenesis.
HostingRepository	PRIDE
AnnounceDate	2025-10-20
AnnouncementXML	Submission_2025-10-19_17:00:55.130.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD057155
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Diego Sbardella
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 240

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-24 08:29:44	ID requested
⏵ 1	2025-10-19 17:00:56	announced

10.1038/s41598-025-19857-z;

10.6019/PXD057155;

Zingale GA, Giammaria S, Pandino I, Placentino L, Ripandelli G, Tundo GR, Grasso G, Bocedi A, Bell PA, Rossi T, Sbardella D, Altered pattern of proteolysis in rhegmatogenous retinal detachment by mining of N-termini datasets from vitreous humor proteome. Sci Rep, 15(1):35848(2025) [pubmed]

submitter keyword: Eye, Vitreous Humor, UHPLC-MS, Ocular Fluids, DDA

M.D. Ph.D. Diego Sbardella
contact affiliation	IRCCS Fondazione Bietti, Rome
contact email	diego.sbardella@fondazionebietti.it
lab head
Diego Sbardella
contact affiliation	IRCCS Fondazione Bietti
contact email	diego.sbardella@fondazionebietti.it
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD057155
     1. Label: PRIDE project
     2. Name: ALTERED PATTERN OF PROTEOLYSIS OF EXTRACELLULAR PROTEINS IN RHEGMATOGENOUS RETINAL DETACHMENT BY MINING OF TRYPTIC AND N-TERMINI DATASETS FROM VITREOUS HUMOR PROTEOME