PXD057128-1

PXD057128 is an original dataset announced via ProteomeXchange.

Title	Probing the Role of O-GlcNAcylation in Modulating Protein-DNA Interactions: Insights from a Proteomic Transcription Factor Response Element-Based Pulldown Screening Approach
Description	O-linked β-N-acetylglucosamine (O-GlcNAc), a critical post-translational modification (PTM) predominantly found in the nucleus, plays a substantial role in regulating gene expression by modulating transcription factors (TFs) activity. Herein, by utilizing a pulldown screening approach termed as tandem array of transcription factors response elements (catTFREs), we unravel the profound impact of fluctuating levels of O-GlcNAcylation on the DNA binding efficiency of endogenous TFs. The proteins were enriched for label-free quantitative proteomics by LC-MS/MS.
HostingRepository	iProX
AnnounceDate	2024-10-24
AnnouncementXML	Submission_2024-10-23_20:10:48.546.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yubo Liu
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-23 20:10:26	ID requested
⏵ 1	2024-10-23 20:10:48	announced

Dataset with its publication pending

submitter keyword: O-GlcNAc, transcription factors, DNA-protein interaction

Yubo Liu
contact affiliation	Dalian University of Technology
contact email	liuyubo@dlut.edu.cn
lab head
Yubo Liu
contact affiliation	Dalian University of Technology
contact email	liuyubo@dlut.edu.cn
dataset submitter

iProX dataset URI