PXD057112 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | TRIM24 directs replicative stress responses to maintain ALT telomeres via chromatin signaling |
| Description | An inability to replicate the genome can cause replication stress and genome instability. Here, we develop BLOCK-ID, a proteomic method to identify and visualize proteins at stressed replication forks. This approach successfully identified novel mediators of the replication stress response, including the chromatin acetylation reader protein TRIM24. In validating TRIM24 function, we uncovered its crucial role in coordinating Alternative Lengthening of Telomeres (ALT), a cancerspecific telomere extension pathway involving replication stress. Our data reveal that TRIM24 is directed to telomeres via a p300/CBP-dependent acetylation chromatin signaling cascade, where it organizes ALT-associated PML bodies (APBs) to promote telomere DNA synthesis. Strikingly, we demonstrate that when artificially tethered at telomeres, TRIM24 can stimulate new telomere DNA synthesis in a SUMO-dependent manner, independently of p300/CBP or PML-dependent APBs. Thus, this study identifies a TRIM24 chromatin signaling pathway required for ALT telomere maintenance. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-08-31 |
| AnnouncementXML | Submission_2026-08-30_16:21:24.415.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | chanhyun na |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | biotinylated residue |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2024-10-23 10:18:55 | ID requested | |
| ⏵ 1 | 2026-08-30 16:21:25 | announced | |
Publication List
| Kim D, Bhargava R, Wang SC, Tseng WC, Lee D, Patel R, Oh S, Bowman RW, Smith BA, Kim M, Na CH, O'Sullivan RJ, Miller KM, TRIM24 directs replicative stress responses to maintain ALT telomeres via chromatin signaling. Mol Cell, 85(14):2636-2653.e8(2025) [pubmed] |
| 10.1016/j.molcel.2025.06.009; |
Keyword List
| submitter keyword: Telomeres,BLOCK-ID,Replication stress,TRIM24,SUMOylation |
Contact List
| Kyle M. Miller |
|---|
| contact affiliation | The University of Texas at Austin |
| contact email | daein.kim@utexas.edu |
| lab head | |
| chanhyun na |
|---|
| contact affiliation | Johns Hopkins University |
| contact email | chanhyun.na@gmail.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD057112 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD057112
- Label: PRIDE project
- Name: TRIM24 directs replicative stress responses to maintain ALT telomeres via chromatin signaling
