PXD057110-1

PXD057110 is an original dataset announced via ProteomeXchange.

Title	Finding the LC3-interactors in senescent human A549 cells during influenza A virus infection
Description	To screen age-related regulators of mitophagy, we examined LC3-interacting proteins by proximity-dependent biotin identification. LC3B was cloned into a mammalian expression vector tagged with a mitochondrial OMM-targeted APEX2 (mito-APEX) label for proximity-based identification of LC3-interacting proteins localized or trafficked to mitochondria during influenza virus infection. Senescent A549 cells, transfected with the mito-APEX-tagged LC3 plasmid for 18 hours, revealed mitochondrial localization of LC3. Following 18 hpi and biotin affinity capture, biotinylated proteins were purified and analyzed by mass spectrometry to identify LC3-interacting proteins.
HostingRepository	PRIDE
AnnounceDate	2025-09-29
AnnouncementXML	Submission_2025-09-28_16:03:38.281.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD057110
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Zhimin Jiang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	4800 Proteomics Analyzer

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-23 07:54:07	ID requested
⏵ 1	2025-09-28 16:03:39	announced

10.6019/PXD057110;

Jiang Z, Pan W, Chen Y, Zhou D, Ren S, Tong Q, Liu L, Sun H, Sun Y, Bi Y, Wang D, Lu L, Pu J, Chang KC, Liu J, ApoD mediates age-associated increase in vulnerability to influenza virus infection. Proc Natl Acad Sci U S A, 122(37):e2423973122(2025) [pubmed]

10.1073/pnas.2423973122;

submitter keyword: Influenza A virus,Lc3, Ifection, Senescent human cells

Zhimin Jiang
contact affiliation	National Key Laboratory of Veterinary Public Health and Safety, Key Laboratory for Prevention and Control of Avian Influenza and Other Major Poultry Diseases, Ministry of Agriculture and Rural Affairs, College of Veterinary Medicine, China Agricultural University, Beijing, China
contact email	jzm@cau.edu.cn
lab head
Zhimin Jiang
contact affiliation	China Agricultural University
contact email	jzm@cau.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/09/PXD057110

PRIDE project URI

• PRIDE
  1. PXD057110
     1. Label: PRIDE project
     2. Name: Finding the LC3-interactors in senescent human A549 cells during influenza A virus infection