PXD057097-1

PXD057097 is an original dataset announced via ProteomeXchange.

Title	Proximity labeling reveals differential interaction partners of the human mitochondrial import receptor proteins TOMM20 and TOMM70
Description	Import of most mitochondrial proteins requires that their precursor proteins are bound by the (peripheral) receptor proteins Tom20, Tom22, and Tom70. For budding yeast Tom20 and Tom70, there is evidence of specific yet overlapping substrate recognition, but no such data is available for metazoan cells. Using APEX2-based proximity labeling, we thus created association profiles for human TOMM20 and TOMM70 in HeLa cells. We particularly focused on their interaction with RNA-binding proteins (RBPs) since there is evidence for RNA association with the mitochondrial outer membrane (MOM) and local translation at the mitochondrial surface, but these processes are poorly understood. Our results show a preferred association of several RBPs and translation factors with TOMM20 over TOMM70. These include SYNJBP2, a previously identified membrane-bound RBP that binds and protects mRNAs encoding mitochondrial proteins, and PAIP1, a translational co-activator interacting with polyA-binding protein. Translational inhibition by puromycin resulted in an even increased association of these RBPs with TOMM20 compared to TOMM70, suggesting that TOMM20 but not TOMM70 might play a role in preserving cellular hemostasis during translation stress by retaining protective RBPs and translation-related proteins at the MOM.
HostingRepository	PRIDE
AnnounceDate	2026-01-07
AnnouncementXML	Submission_2026-01-07_03:19:06.381.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD057097
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Bianca Lemke
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-23 03:39:24	ID requested
⏵ 1	2026-01-07 03:19:07	announced

10.1021/ACS.JPROTEOME.5C00905;

10.6019/PXD057097;

submitter keyword: Human, TOMM20, LC-MS, proximity labeling, SYNJ2BP, localized translation, TOMM70

Ralf-Peter Jansen
contact affiliation	Interfaculty Institute of Biochemistry, University of Tübingen, Tübingen, Germany
contact email	ralf.jansen@uni-tuebingen.de
lab head
Bianca Lemke
contact affiliation	University of Tuebingen
contact email	bianca.lemke@uni-tuebingen.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD057097
     1. Label: PRIDE project
     2. Name: Proximity labeling reveals differential interaction partners of the human mitochondrial import receptor proteins TOMM20 and TOMM70