PXD057034-1

PXD057034 is an original dataset announced via ProteomeXchange.

Title	Human zinc binding cysteine proteome
Description	Zinc is an essential micronutrient that regulates a wide range of physiological processes, principally through Zn2+ binding to protein cysteine residues. Despite being critical for modulation of protein function, for the vast majority of the human proteome the cysteine sites subject to Zn2+ binding remain undefined. Here we develop ZnCPT, a deep and quantitative mapping of the zinc binding cysteine proteome. We define 6173 zinc binding protein cysteines, uncovering protein families across major domains of biology that are subject to either constitutive or inducible zinc binding. ZnCPT enables systematic discovery of zinc-regulated structural, enzymatic, and allosteric functional domains. On this basis, we identify 52 cancer genetic dependencies subject to zinc binding, and nominate malignancies sensitive to zinc-induced cytotoxicity. In doing so, we discover a mechanism of zinc regulation over Glutathione Reductase (GSR) that drives cell death in GSR-dependent lung cancers. We provide ZnCPT as a resource for understanding mechanisms of zinc regulation of protein function.
HostingRepository	PRIDE
AnnounceDate	2025-01-03
AnnouncementXML	Submission_2025-01-03_08:53:34.433.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nils Burger
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	L-methionine sulfoxide
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-21 16:32:16	ID requested
⏵ 1	2025-01-03 08:53:34	announced

10.1016/J.CELL.2024.11.025;

submitter keyword: CPT-based cysteines enrichment, Prostate, HCT116, Liver,Zinc

Edward Chouchani
contact affiliation	Dana-Farber Cancer Institute Harvard Medical School
contact email	EdwardT_Chouchani@dfci.harvard.edu
lab head
Nils Burger
contact affiliation	Dana-Farber Cancer Institute and Harvard Medical School
contact email	Nils_Burger@dfci.harvard.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/01/PXD057034

PRIDE project URI

• PRIDE
  1. PXD057034
     1. Label: PRIDE project
     2. Name: Human zinc binding cysteine proteome