PXD057032-1

PXD057032 is an original dataset announced via ProteomeXchange.

Title	Phosphoproteome enrichment strategy using complementary chemistries of Fe-NTA and TiO2 followed by LC-MS/MS
Description	The global phosphoproteome analysis by liquid chromatography-mass spectrometry (LC-MS) has emerged as an important tool to study cell signalling. However, the coverage of phosphoproteome by LC-MS is limited despite various approaches for phosphoprotein enrichment. Studies in literature have exploited the complementary chemistries of affinity materials like metal (immobilized metal ion affinity chromatography- IMAC) and metal oxides (metal oxide affinity chromatography- MOAC) independently and in a sequential elution approach to improve coverage of phosphoproteins. We demonstrate that further improvement in the enrichment of phosphoproteins can be achieved by using these metals and metal oxides independently and pooling the data.
HostingRepository	PRIDE
AnnounceDate	2025-10-30
AnnouncementXML	Submission_2025-10-29_23:49:05.198.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Neha Agrawal
SpeciesList	scientific name: Danio rerio (Zebrafish) (Brachydanio rerio); NCBI TaxID: NEWT:7955; scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: NEWT:7227; scientific name: Bombyx mori (Silk moth); NCBI TaxID: NEWT:7091; scientific name: Brassica napus (Rape); NCBI TaxID: NEWT:3708; scientific name: Candida albicans (Yeast); NCBI TaxID: NEWT:5476; scientific name: Caenorhabditis elegans; NCBI TaxID: NEWT:6239; scientific name: Dictyostelium discoideum (Slime mold); NCBI TaxID: NEWT:44689; scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; scientific name: Cercopithecus aethiops (Green monkey) (Grivet); NCBI TaxID: NEWT:9534; scientific name: Brassica oleracea var. botrytis (Cauliflower); NCBI TaxID: NEWT:3715; scientific name: Cicer arietinum (Chickpea) (Garbanzo); NCBI TaxID: NEWT:3827; scientific name: Bos taurus (Bovine); NCBI TaxID: NEWT:9913; scientific name: Pinus radiata (Monterey pine); NCBI TaxID: NEWT:3347;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos; LCQ Classic; TripleTOF 5600; Q Exactive; 4700 Proteomics Analyzer; 4800 Proteomics Analyzer; LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-21 15:52:43	ID requested
⏵ 1	2025-10-29 23:49:05	announced

Dataset with its publication pending

submitter keyword: affinity chromatography,Phosphoproteins, enrichment, LC-MS/MS

Dr Rukmini Govekar
contact affiliation	ACTREC, TMC, India
contact email	rgovekar@actrec.gov.in
lab head
Neha Agrawal
contact affiliation	Advanced Centre for Treatment, Research and Education in Cancer
contact email	nagrawal9528@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/10/PXD057032

PRIDE project URI

• PRIDE
  1. PXD057032
     1. Label: PRIDE project
     2. Name: Phosphoproteome enrichment strategy using complementary chemistries of Fe-NTA and TiO2 followed by LC-MS/MS