PXD057026-1

PXD057026 is an original dataset announced via ProteomeXchange.

Title	Absence of MCJ/DnaJC15 promotes brown adipose tissue thermogenesis
Description	Obesity poses a global health challenge, demanding a deeper understanding of adipose tissue (AT) and its mitochondria. This study describes the role of the mitochondrial protein Methylation-controlled J protein (MCJ/DnaJC15) in orchestrating brown adipose tissue (BAT) thermogenesis. Here we show how MCJ expression decreases during obesity, as evident in human and mouse adipose tissue samples. MCJKO mice, even without UCP1, a fundamental thermogenic protein, exhibit elevated BAT thermogenesis. Electron microscopy unveils changes in mitochondrial morphology resembling BAT activation. Proteomic analysis confirms these findings and suggests involvement of the eIF2α mediated stress response. The pivotal role of eIF2α is scrutinized by in vivo CRISPR deletion of eIF2α in MCJKO mice, abrogating thermogenesis. These findings uncover the importance of MCJ as a regulator of BAT thermogenesis, presenting it as a promising target for obesity therapy.
HostingRepository	PRIDE
AnnounceDate	2024-11-13
AnnouncementXML	Submission_2024-11-13_07:49:19.964.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Juan A Lopez
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-21 13:14:31	ID requested
⏵ 1	2024-11-13 07:49:20	announced

Dataset with its publication pending

submitter keyword: Proeomics,Mouse, Lipidomics, adipose tissue

Juan A Lopez
contact affiliation	Proteomics Unit, CNIC; Madrid, Spain
contact email	jalopez@cnic.es
lab head
Juan A Lopez
contact affiliation	CNIC
contact email	jalopez@cnic.es
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD057026
     1. Label: PRIDE project
     2. Name: Absence of MCJ/DnaJC15 promotes brown adipose tissue thermogenesis