PXD057016-1

PXD057016 is an original dataset announced via ProteomeXchange.

Title	proximity labeling BSA through PCN
Description	For the liquid chromatography-mass spectrometry (LC-MS) analysis, the 500 μM AP was used to label BSA in the reaction, followed by clicking chemical conjugation with 0.1 mM azide-PEG3-biotin under 0.5 mM CuCl(2), 2 Mm Tris (3-hydroxypropyltriazolylmethyl) amine (THPTA, Sigma-Aldrich) and 5 mM ascorbic acid. Then, the identification of the labeled site was analyzed by a HF-X mass spectrometer (ThermoFisher) that was coupled to a nano Elute liquid chromatography system (Bruker Daltonics). MS data were processed by the Byonic software 4.0.12 version (Protein Metrics INC.) for both de novo sequencing and database search. The peptides with scores higher than 200 were retained for analysis.
HostingRepository	PRIDE
AnnounceDate	2025-07-30
AnnouncementXML	Submission_2025-07-30_00:15:43.460.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zihan Wang
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: 9913;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-21 07:30:20	ID requested
⏵ 1	2025-07-30 00:15:43	announced
2	2025-09-19 06:19:12	announced	2025-09-19: Updated project metadata.

Dataset with its publication pending

submitter keyword: PCN BSA

Han shuo
contact affiliation	SIBCB
contact email	Wangzihan2023@sibcb.ac.cn
lab head
Zihan Wang
contact affiliation	SIBCB
contact email	wangzihan2023@sibcb.ac.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/07/PXD057016

PRIDE project URI

• PRIDE
  1. PXD057016
     1. Label: PRIDE project
     2. Name: proximity labeling BSA through PCN