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PXD057000-1

PXD057000 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDDX5 competes with ZC3H12D regulating CCND1 transcript stability to modulate cell cycle progression in breast cancer
DescriptionThis study was performed according to protein pull-down assay using GFP-coated beads in MDA-MB-468 cells expressing GFP/ZC3H12D fusion protein, and then the precipitated proteins were isolated by running SDS-PAGE and silver staining, and identified by high resolution mass spectrometry.This study was performed according to RNA pull-down assay using biotin-labeled WT RNA probe (with stem-loop structure) or mutant RNA probe (without stem-loop structure) and streptavidin-coated Dynabeads in human breast tumor cell MDA-MB-468, and then the pulled down proteins were isolated by SDS-PAGE and silver staining, and identified by high resolution mass spectrometry.
HostingRepositoryiProX
AnnounceDate2024-10-21
AnnouncementXMLSubmission_2024-10-21_01:22:32.632.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterWenbao Lu
SpeciesList scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-10-21 01:22:11ID requested
12024-10-21 01:22:33announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Interacting proteins with ZC3H12D in breast tumor cell MDA-MB-468, protein pull-down followed by MS.Assosiated proteins with CCND1 RNA stem-loop structure in breast tumor cell MDA-MB-468, RNA pull-down followed by MS.
Contact List
Wenbao Lu
contact affiliationChinese Academy of Medical Sciences & Peking Union Medical College
contact emailluwenbao_217@imc.pumc.edu.cn
lab head
Wenbao Lu
contact affiliationChinese Academy of Medical Sciences & Peking Union Medical College
contact emailluwenbao_217@imc.pumc.edu.cn
dataset submitter
Full Dataset Link List
iProX dataset URI