PXD056979-1

PXD056979 is an original dataset announced via ProteomeXchange.

Title	Diesel particulate matter-induced proteomic changes in three-dimensional spheroids derived from human primary cells of various tissue origins
Description	This study investigated the effects of diesel particulate matter (DPM) on multiple human organs using 3D spheroids derived from eight human primary cell types. To assess the impact of DPM, we exposed these spheroids to varying concentrations of standardized DPM (SRM 2975) and measured their viability, followed by proteomic analysis using tandem mass tag (TMT) labeling with LC-MS/MS. A total of 9,707 proteins were identified, with 128 proteins exhibiting statistically significant changes (P-value < 0.05) in response to DPM exposure, as determined by two-way ANOVA. Among these, five proteins, including apolipoprotein A-I (APOA1), significantly increased at higher DPM concentrations, while 36 proteins, primarily ribosomal proteins, showed notable decreases even at lower DPM levels. Canonical pathway analysis revealed activation of acute phase response signaling, LXR/RXR, and FXR/RXR pathways across all spheroid types. APOA1 was identified as a potential biomarker for DPM exposure, with increased expression potentially linked to disruption in protein degradation pathways. This study provides valuable insights into the systemic toxicity of DPM, highlighting key proteomic changes across different tissue types and identifying potential biomarkers that could be used to assess exposure levels and health risks related to particulate matter.
HostingRepository	PRIDE
AnnounceDate	2025-08-22
AnnouncementXML	Submission_2025-08-21_20:24:55.630.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ji Eun Lee
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-20 12:18:31	ID requested
⏵ 1	2025-08-21 20:24:56	announced

Dataset with its publication pending

submitter keyword: three-dimensional (3D) spheroids, mass spectrometry (MS), diesel particulate matter (DPM), viability assessment, tandem mass tag (TMT)

Ji Eun Lee
contact affiliation	Korea Institue of Scicence and Technology
contact email	jelee9137@kist.rek.kr
lab head
Ji Eun Lee
contact affiliation	Korea Institue of Science and Technology
contact email	jelee9137@kist.re.kr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD056979
     1. Label: PRIDE project
     2. Name: Diesel particulate matter-induced proteomic changes in three-dimensional spheroids derived from human primary cells of various tissue origins