PXD056971-1

PXD056971 is an original dataset announced via ProteomeXchange.

Title	Proximity proteomics provides a new resource for exploring the function of Afadin and the complexity of cell-cell adherens junctions
Description	Afadin and its Drosophila homolog Canoe are key components of the network of proteins that help link cell-cell adherens junctions to the actomyosin cytoskeleton. They stabilize this connection as force is generated to drive cell shape change and migration during embryonic morphogenesis, organogenesis and tissue homeostasis. Some domains or regions have known binding partners, but the interactors for other well conserved domains remain unknown. We sought an unbiased approach to identify proteins within the Afadin protein network, including those that bind directly and those that are proximal. To do so, we made use of BioID-based proximity labeling, in which the promiscuous biotin ligase enzyme (BirA-R118G, referred to as BirA*) is fused to a protein of interest. Following the addition of exogenous biotin to live cells expressing the BirA*:bait fusion, proteins within a ~10-50nM sphere are biotinylated. Biotinylated proximal proteins are then purified with streptavidin for downstream identification and quantification.
HostingRepository	PRIDE
AnnounceDate	2025-01-20
AnnouncementXML	Submission_2025-01-20_08:51:51.163.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dennis Goldfarb
SpeciesList	scientific name: Canis familiaris (Dog) (Canis lupus familiaris); NCBI TaxID: 9615;
ModificationList	biotinylated residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-19 11:58:14	ID requested
⏵ 1	2025-01-20 08:51:52	announced

10.1101/2024.11.07.622507;

Choi W, Goldfarb D, Yan F, Major MB, Fanning AS, Peifer M, Proximity proteomics provides a new resource for exploring the function of Afadin and the complexity of cell-cell adherens junctions. bioRxiv, ():(2024) [pubmed]

submitter keyword: BioID, Afadin, cell-cell adherens junctions

Mark Peifer
contact affiliation	Department of Biology Lineberger Comprehensive Cancer Center University of North Carolina at Chapel Hill
contact email	peifer@unc.edu
lab head
Dennis Goldfarb
contact affiliation	Cell Biology and Physiology Institute for Informatics
contact email	d.goldfarb@wustl.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/01/PXD056971

PRIDE project URI

• PRIDE
  1. PXD056971
     1. Label: PRIDE project
     2. Name: Proximity proteomics provides a new resource for exploring the function of Afadin and the complexity of cell-cell adherens junctions