PXD056946-1

PXD056946 is an original dataset announced via ProteomeXchange.

Title	The dual life of disordered lysine-rich domains of snoRNPs in rRNA modification and nucleolar compaction
Description	Intrinsically disordered regions (IDRs) are highly enriched in the nucleolar proteome but their physiological role in ribosome assembly remains poorly understood. Our study reveals the functional plasticity of the extremely abundant lysine-rich IDRs of small nucleolar ribonucleoprotein particles (snoRNPs) from protists to mammalian cells. We show in Saccharomyces cerevisiae that the electrostatic properties of this lysine-rich IDR, the KKE/D domain, promote snoRNP accumulation in the vicinity of nascent rRNAs, facilitating their modification. Under stress conditions reducing the rate of ribosome assembly, they are essential for nucleolar compaction and sequestration of key early-acting ribosome biogenesis factors, including RNA polymerase I, owing to their self-interaction capacity in a latent, non-rRNA-associated state. We propose that such functional plasticity of these lysine-rich IDRs may represent an ancestral eukaryotic regulatory mechanism, explaining how nucleolar morphology is continuously adapted to rRNA production levels.
HostingRepository	PRIDE
AnnounceDate	2024-10-18
AnnouncementXML	Submission_2024-10-18_07:39:28.605.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michael Stumpe
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-18 06:19:09	ID requested
⏵ 1	2024-10-18 07:39:29	announced

Dataset with its publication pending

submitter keyword: snoRNP,lysine-rich IDR, Saccharomyces cerevisiae

Dieter Kressler
contact affiliation	Dept. of Biology University of Fribourg Chemin du Musee 10 CH-1700 Fribourg
contact email	dieter.kressler@unifr.ch
lab head
Michael Stumpe
contact affiliation	Department of Biology, University of Fribourg
contact email	michael.stumpe@unifr.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/10/PXD056946

PRIDE project URI

• PRIDE
  1. PXD056946
     1. Label: PRIDE project
     2. Name: The dual life of disordered lysine-rich domains of snoRNPs in rRNA modification and nucleolar compaction