PXD056941-1

PXD056941 is an original dataset announced via ProteomeXchange.

Title	MYC deregulation sensitizes cancer cells to N-myristoyltransferase inhibition - Mitochondrial fractionation
Description	Human N-myristoyltransferases (NMTs) catalyze N-terminal protein N myristoylation and are promising targets in cancer, with an emerging mechanistic rationale for targeted therapy. Here, we screened 245 cancer cell lines against IMP-1320, a potent NMT inhibitor (NMTi), and conduced pathway-level analyses to identify that deregulated MYC increases cancer cell sensitivity to NMTi. Proteomics on detergent enriched membrane fractions in MYC or MYCN deregulated cancer cell models revealed that cell death is associated at least in part with loss of membrane association of mitochondrial respiratory complex I. This is concurrent with loss of myristoylation and degradation of the complex I assembly factor NDUFAF4, and induction of mitochondrial dysfunction, driven by MYC or MYCN deregulation. NMTi eliminated or suppressed MYC and MYCN-driven tumors in vivo without overt toxicity, suggesting that this constitutive co translational protein modification can be targeted in MYC driven cancers.
HostingRepository	PRIDE
AnnounceDate	2025-07-30
AnnouncementXML	Submission_2025-07-30_02:28:52.790.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wouter Kallemeijn
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-18 04:15:00	ID requested
⏵ 1	2025-07-30 02:28:53	announced

Dataset with its publication pending

submitter keyword: N-myristoyltransferase, proteomics, mitochondrial fractionation,N-Myristoylation

Professor Ed Tate
contact affiliation	GSK Chair in Chemical Biology, Imperial College London Satellite Group Leader, Francis Crick Institute Founder & SAB, Myricx Bio (https://myricxbio.com/) Room 301B, Molecular Sciences Research Hub, White City Campus 82 Wood Lane, London W12 0BZ, UK Tel.: 020759 + Ext. 43752 or 45821 (group) http://www.imperial.ac.uk/tate-group/
contact email	e.tate@imperial.ac.uk
lab head
Wouter Kallemeijn
contact affiliation	The Francis Crick Institute and Imperial College London
contact email	wouter.kallemeijn@crick.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/07/PXD056941

PRIDE project URI

• PRIDE
  1. PXD056941
     1. Label: PRIDE project
     2. Name: MYC deregulation sensitizes cancer cells to N-myristoyltransferase inhibition - Mitochondrial fractionation