PXD056927-1

PXD056927 is an original dataset announced via ProteomeXchange.

Title	Cell-autonomous targeting of arabinogalactan by host immune factors inhibits mycobacterial growth
Description	Deeper understanding of the crosstalk between host cells and Mycobacterium tuberculosis (Mtb) provides crucial guidelines for the rational design of novel intervention strategies against tuberculosis (TB). Mycobacteria possess a unique complex cell wall with arabinogalactan (AG) as critical component. AG has been identified as a virulence factor of Mtb which is recognized by host galectin-9. Here we demonstrate that galectin-9 directly inhibited mycobacterial growth through AG-binding property of carbohydrate-recognition domain 2. Furthermore, IgG antibodies with AG specificity were detected in serum of TB patients. Based on the interaction between galectin-9 and AG, we developed monoclonal antibody (mAb) screening assay and identified AG-specific mAbs which profoundly inhibit Mtb growth. Mechanistically, proteomic profiling and morphological characterizations revealed that AG-specific mAbs regulate AG biosynthesis, thereby inducing cell wall swelling. Thus, direct AG-binding by galectin-9 or antibodies contributes to protection against TB. Our findings pave the way for the rational design of novel immunotherapeutic strategies for TB control.
HostingRepository	PRIDE
AnnounceDate	2024-10-18
AnnouncementXML	Submission_2024-10-18_00:19:41.162.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jianxia Chen
SpeciesList	scientific name: Mycobacterium tuberculosis NA-A0009; NCBI TaxID: 1200348;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-17 20:23:53	ID requested
⏵ 1	2024-10-18 00:19:41	announced

10.7554/ELIFE.92737.2;

submitter keyword: antibody,Mycobacterium tuberculosis, tuberculosis, galectin-9, arabinogalactan

Haipeng Liu
contact affiliation	Shanghai Pulmonary Hospital 1Shanghai Key Laboratory of Tuberculosis, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai 200433, PR China 2Department of Microbiology and Immunology, Tongji University School of Medicine, Shanghai 200049, PR China
contact email	haipengliu@tongji.edu.cn
lab head
Jianxia Chen
contact affiliation	Shanghai Pulmonary Hospital
contact email	jxchen01@sibs.ac.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/10/PXD056927

PRIDE project URI

• PRIDE
  1. PXD056927
     1. Label: PRIDE project
     2. Name: Cell-autonomous targeting of arabinogalactan by host immune factors inhibits mycobacterial growth