PXD056912-1

PXD056912 is an original dataset announced via ProteomeXchange.

Title	tyrosine kinase oxidation by gas plasma
Description	TKs (tyrosine kinases) dissolved in liquids were exposed to gas plasma, and a drastic reduction of their activity was observed. Hypothesizing that this was due to gas plasma-generated ROS, plasma-treated TKs were analyzed by high-resolution mass spectrometry for the type and quantity of oxPTM types using an in-house database. Preferred oxidation targets were identified as sulfur-containing and aromatic amino acids. OxPTMs were detected on amino acid residues that have important structural or catalytic functions in TKs, such as the ATP binding site, but also on amino acid residues that are targets for therapeutic applications, such as TK inhibitors. TK dissolved in liquids were exposed to gas plasma, and a drastic reduction of their activity was observed. Hypothesizing that this was due to gas plasma-generated ROS, plasma-treated TKs were analyzed by high-resolution mass spectrometry for the type and quantity of oxPTM types using an in-house database. Preferred oxidation targets were identified as sulfur-containing and aromatic amino acids. OxPTMs were detected on amino acid residues that have important structural or catalytic functions in TKs, such as the ATP binding site, but also on amino acid residues that are targets for therapeutic applications, such as TK inhibitors.
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-07_04:21:51.394.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD056912
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Kristian Wende
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	dihydroxylated residue; dehydrated residue; monohydroxylated residue; deamidated residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-17 09:42:19	ID requested
⏵ 1	2025-05-07 04:21:52	announced

10.1116/6.0003970;

10.6019/PXD056912;

Schulan P, Wende K, von Woedtke T, Weltmann KD, Bekeschus S, Clemen R, Oxidative modifications control aberrant tyrosine kinase activity. Biointerphases, 19(6):(2022) [pubmed]

submitter keyword: receptor tyrosin kinase, RNS, ROS,non thermal plasma

Kristian Wende
contact affiliation	Leibniz Institute for Plasma Science and Technogy
contact email	kristian.wende@inp-greifswald.de
lab head
Kristian Wende
contact affiliation	Leibniz Institute for Plasma Science and Technogy
contact email	kristian.wende@inp-greifswald.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD056912
     1. Label: PRIDE project
     2. Name: tyrosine kinase oxidation by gas plasma