PXD056895-1

PXD056895 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Oxygen-confined photooxidation represents a novel form of oxidative protein damage
Description	Oxidative modifications can disrupt protein folds and functions, and are strongly associated with human aging and diseases. Conventional oxidation pathways typically involve the free diffusion of reactive oxygen species (ROS), which primarily attack the protein surface. Yet, it remains unclear whether and how internal protein folds capable of trapping oxygen (O2) contribute to oxidative damage. Here, we report a novel pathway of protein damage, which we refer to asO2-confined photooxidation. In this process, O2 is captured in protein cavities and subsequently converted into multiple ROS, primarily mediated by tryptophan residues under blue light irradiation. The generated ROS then attack the protein interior through constrained diffusion, causing protein damage. The effects of this photooxidative reaction appear to be extensive, impacting a wide range of cellular proteins,as supported by whole-cell proteomic analysis. This photooxidative mechanism may represent a latent oxidationpathway in human tissuesdirectly exposed to visible light, such as skin and eyes.
HostingRepository	jPOST
AnnounceDate	2024-11-27
AnnouncementXML	Submission_2024-11-26_21:43:42.204.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Seungjin Na
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; unknown modification; unknown modification; unknown modification; unknown modification; L-kynurenine
Instrument	Orbitrap Fusion Lumos; Orbitrap Eclipse

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-17 03:50:48	ID requested
⏵ 1	2024-11-26 21:43:42	announced
2	2024-12-31 18:38:16	announced	2025-01-01: Updated PubMed.

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: oxidative protein damage, oxygen confinement, protein cavity, ROS generation, protein interior oxidation, photooxidation

submitter keyword: oxidative protein damage, oxygen confinement, protein cavity, ROS generation, protein interior oxidation, photooxidation

Contact List

Duyoung Min
lab head
Seungjin Na
contact affiliation	Korea Basic Science Institute
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST003425/