PXD056894-1

PXD056894 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Towards sensitive, streamlined high throughput protein thermal stability analysis for low sample input
Description	This method development aims to enhance and streamline excisting PISA workflows by reducing pipetting steps and digestion method. With this workflow we want to provide a method with higher reproducibility and simplicity. This method combines the excisting OneTip protocol with low cell concentration input on a 96 well format to reach a high kinase inhibitor target screening throughput from treatment to sample injection within 24h.
HostingRepository	PRIDE
AnnounceDate	2025-10-07
AnnouncementXML	Submission_2025-10-07_01:35:38.523.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Maico Yannic Lechner
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Astral

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-17 02:15:10	ID requested
⏵ 1	2025-10-07 01:35:39	announced

Publication List

Lechner M, Sabatier P, Olsen JV, High-throughput proteome integral solubility alteration assay for low cell input using One-Tip. Commun Chem, 8(1):282(2025) [pubmed]
10.1038/s42004-025-01670-4;

Lechner M, Sabatier P, Olsen JV, High-throughput proteome integral solubility alteration assay for low cell input using One-Tip. Commun Chem, 8(1):282(2025) [pubmed]

10.1038/s42004-025-01670-4;

Keyword List

submitter keyword: LC-MS, OneTip, Method development, Proteomics, PISA

submitter keyword: LC-MS, OneTip, Method development, Proteomics, PISA

Contact List

Jesper Velgaard Olsen
contact affiliation	Novo Nordisk Foundation Center for Protein Research
contact email	jesper.olsen@cpr.ku.dk
lab head
Maico Yannic Lechner
contact affiliation	NNF CPR
contact email	maico.lechner@cpr.ku.dk
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/10/PXD056894
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/10/PXD056894

PRIDE project URI

Repository Record List

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