PXD056847-1

PXD056847 is an original dataset announced via ProteomeXchange.

Title	The Role of Core Virulence Kinase Bud32 in Iron Homeostasis Regulation in Cryptococcus neoformans
Description	The core virulence kinase Bud32, which is critical for lung and brain infections, influences the production of major virulence factors in Cryptococcus neoformans, including capsule formation, melanin production, and growth at 37°C. This study explores the role of Bud32 in regulation of iron homeostasis in C. neoformans. Bud32 was required for growth under iron limiting condition. Loss of Bud32 resulted in significant alterations in the expression of proteins related to iron binding and iron-sulfur cluster assembly. Moreover, metabolic profiling identified 696 metabolites that were up-regulated and 480 that were down-regulated in the bud32 deletion mutant, highlighting the significant impact of Bud32 loss on intracellular metabolite levels. In particular, the levels of biliverdin, which is primarily derived from heme, showed a significant decrease in the absence of Bud32. Furthermore, Bud32 has been demonstrated to modulate iron-sulfur cluster assembly, likely influencing the interaction between the iron-sulfur binding protein Grx4 and the iron regulatory factors Cir1 and HapX. Phosphorylation analysis revealed that the deletion of Bud32 caused widespread changes in the phosphorylation status of various proteins, including the iron regulatory factors Cir1 and Rim101. These findings indicate that the Bud32 kinase plays a vital role in regulating iron homeostasis in C. neoformans.
HostingRepository	PRIDE
AnnounceDate	2025-07-23
AnnouncementXML	Submission_2025-07-23_01:12:55.629.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Peng Xue
SpeciesList	scientific name: Cryptococcus neoformans var. grubii serotype A (strain H99 / ATCC 208821 / CBS 10515 / FGSC 9487) (Filobasidiella neoformans var. grubii); NCBI TaxID: 235443;
ModificationList	phosphorylated residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-16 01:49:26	ID requested
⏵ 1	2025-07-23 01:12:56	announced

10.3389/FIMMU.2025.1624237;

submitter keyword: Bud32

Grx4

Cir1

HapX

Rim101

Peng Xue
contact affiliation	Nantong Key Laboratory of Environmental Toxicology, Department of Occupational Medicine and Environmental Toxicology, School of Public Health, Nantong University, Nantong, China
contact email	pengxue@ntu.edu.cn
lab head
Peng Xue
contact affiliation	https://www.ebi.ac.uk/pride/
contact email	pengxue@ntu.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD056847
     1. Label: PRIDE project
     2. Name: The Role of Core Virulence Kinase Bud32 in Iron Homeostasis Regulation in Cryptococcus neoformans