PXD056836-1

PXD056836 is an original dataset announced via ProteomeXchange.

Title	PTPN23-dependent ESCRT machinery functions as a cell death checkpoint in restraining multiple cell death pathways
Description	In our study, a CRISPR screen of acute myeloid leukemia cells identifies protein tyrosine phosphatase non-receptor type 23 (PTPN23) as essential for survival. Loss of PTPN23 activates nuclear factor-kappa B, apoptotic, necroptotic, and pyroptotic pathways by causing the accumulation of death receptors and toll-like receptors (TLRs) in endosomes. These effects are recapitulated by depletion of PTPN23 co-dependent genes in the endosomal sorting complex required for transport (ESCRT) pathway. Through proximity-dependent biotin labeling, we show that NAK-associated protein 1 interacts with PTPN23 to facilitate endosomal sorting of tumor necrosis factor receptor 1 (TNFR1), sensitizing cells to TNF--induced cytotoxicity. Our findings reveal PTPN23-dependent ESCRT machinery as a cell death checkpoint that regulates the spatiotemporal distribution of death receptors and TLRs to restrain multiple cell death pathways.
HostingRepository	iProX
AnnounceDate	2024-10-15
AnnouncementXML	Submission_2024-11-17_18:05:37.259.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dongyan Song
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-15 21:56:43	ID requested
⏵ 1	2024-11-17 18:05:37	announced
2	2024-12-04 21:22:54	announced	2024-12-05: Update information.

Dataset with its publication pending

submitter keyword: PTPN23, BioID

Nicholas K. Tonks
contact affiliation	Cold Spring Harbor Laboratory
contact email	tonks@cshl.edu
lab head
Dongyan Song
contact affiliation	Cold Spring Harbor Laboratory
contact email	dongyan.song.cn@gmail.com
dataset submitter

iProX dataset URI