PXD056836
PXD056836 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | PTPN23-dependent ESCRT machinery functions as a cell death checkpoint in restraining multiple cell death pathways |
| Description | In our study, a CRISPR screen of acute myeloid leukemia cells identifies protein tyrosine phosphatase non-receptor type 23 (PTPN23) as essential for survival. Loss of PTPN23 activates nuclear factor-kappa B, apoptotic, necroptotic, and pyroptotic pathways by causing the accumulation of death receptors and toll-like receptors (TLRs) in endosomes. These effects are recapitulated by depletion of PTPN23 co-dependent genes in the endosomal sorting complex required for transport (ESCRT) pathway. Through proximity-dependent biotin labeling, we show that NAK-associated protein 1 interacts with PTPN23 to facilitate endosomal sorting of tumor necrosis factor receptor 1 (TNFR1), sensitizing cells to TNF--induced cytotoxicity. Our findings reveal PTPN23-dependent ESCRT machinery as a cell death checkpoint that regulates the spatiotemporal distribution of death receptors and TLRs to restrain multiple cell death pathways. |
| HostingRepository | iProX |
| AnnounceDate | 2024-10-15 |
| AnnouncementXML | Submission_2024-12-04_21:22:53.248.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Dongyan Song |
| SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Fusion |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2024-10-15 21:56:43 | ID requested | |
| 1 | 2024-11-17 18:05:37 | announced | |
| ⏵ 2 | 2024-12-04 21:22:54 | announced | 2024-12-05: Update information. |
Publication List
| Song D, Cen Y, Qian Z, Wu XS, Rivera K, Wee TL, Demerdash OE, Chang K, Pappin D, Vakoc CR, Tonks NK, PTPN23-dependent ESCRT machinery functions as a cell death checkpoint. Nat Commun, 15(1):10364(2024) [pubmed] |
Keyword List
| submitter keyword: PTPN23, BioID |
Contact List
| Nicholas K. Tonks | |
|---|---|
| contact affiliation | Cold Spring Harbor Laboratory |
| contact email | tonks@cshl.edu |
| lab head | |
| Dongyan Song | |
| contact affiliation | Cold Spring Harbor Laboratory |
| contact email | dongyan.song.cn@gmail.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
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