⮝ Full datasets listing
PXD056823-1
PXD056823 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Circadian clock control of ribosome composition promotes rhythmic translation and termination fidelity |
| Description | We describe circadian clock-dependent changes in ribosome composition in Neurospora crassa depending on cell type, stress, or developmental state. Mass spectrometry of ribosomes isolated at different circadian times identified six ribosomal proteins and one ribosome-associated protein with clock-controlled abundance. We confirmed clock control of eL31-HA abundance in purified ribosomes and found that deletion of eL31 altered and inhibited translation rhythms. We examined ribosome protected footprint (RPF)-seq reads mapping past stop codons over circadian time to reveal clock-dependent and eL31-enhanced rhythms in translation termination fidelity. We discovered that eL31 promotes proper ion homeostasis and translation elongation fidelity, and demonstrated that the circadian clock governs daily changes in ribosome composition that control rhythms in translation and impact translation fidelity, likely through changes in intracellular Mg2+ levels. |
| HostingRepository | MassIVE |
| AnnounceDate | 2025-10-29 |
| AnnouncementXML | Submission_2025-10-29_15:33:28.795.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Matthew Monroe |
| SpeciesList | scientific name: Neurospora crassa; NCBI TaxID: 5141; |
| ModificationList | Dehydro; Acetyl; Methyl; Dimethyl; Trimethyl; Phospho |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2024-10-15 11:10:38 | ID requested | |
| ⏵ 1 | 2025-10-29 15:33:29 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: circadian clock, ribosome composition, rhythmic translation, translation fidelity, large ribosomal protein eL31, DatasetType:Proteomics |
Contact List
| Deborah Bell-Pedersen | |
|---|---|
| contact affiliation | Department of Biology, Texas A&M University |
| contact email | dpedersen@tamu.edu |
| lab head | |
| Matthew Monroe | |
| contact affiliation | Pacific Northwest National Laboratory |
| contact email | matthew.monroe@pnnl.gov |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v08/MSV000096101/ |
