PXD056798-1

PXD056798 is an original dataset announced via ProteomeXchange.

Title	Comprehensive Proteomic Analysis Reveals Novel Antibiotic Resistance Proteins and Biomarkers in Multidrug-Resistant in Klebsiella pneumoniae Clinical Isolates
Description	The emergence of multidrug-resistant (MDR) Klebsiella pneumoniae represents a major public health concern, primarily driven by its ability to evade a wide range of antibiotics. Despite extensive genomic studies, proteomic insights into antibiotic resistance mechanisms remain scarce. Here, we employed a data-independent acquisition (DIA)-based quantitative proteomics approach to investigate proteomic differences between 87 MDR and 20 antibiotic-sensitive K. pneumoniae clinical isolates. A total of 3,380 proteins were identified, with 896 showing significant differential expression. MDR isolates exhibited increased expression of efflux pumps, beta-lactamases, and transcriptional regulators, while proteins associated with glycerolipid metabolism and transport were enriched in sensitive strains. To validate our findings, an independent cohort of 10 MDR and 11 sensitive isolates was analyzed. Key biomarkers identified in the discovery cohort, including pyruvate decarboxylase and aldehyde dehydrogenase, were validated with high discriminatory power (AUC > 0.85) in the validation cohort. These findings provide novel insights into the molecular mechanisms of antibiotic resistance and identify promising biomarkers for diagnosing MDR K. pneumoniae, offering potential avenues for therapeutic intervention.
HostingRepository	iProX
AnnounceDate	2024-10-14
AnnouncementXML	Submission_2024-10-14_20:26:55.529.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Guibin Wang
SpeciesList	scientific name: Klebsiella pneumoniae JH1; NCBI TaxID: 1037890;
ModificationList	iodoacetamide derivatized residue; methionine oxidation with neutral loss of 64 Da; acetylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-14 20:26:25	ID requested
⏵ 1	2024-10-14 20:26:55	announced

Dataset with its publication pending

submitter keyword: Proteomics, Klebsiella pneumoniae, Biomarkers, Drug resistance mechanisms, Efflux transporters

Xiangmin Lin
contact affiliation	Fujian Agriculture and Forestry University)
contact email	xiangmin@fafu.edu.cn
lab head
Guibin Wang
contact affiliation	National Center for Protein Sciences (Beijing)
contact email	wangguibin85@163.com
dataset submitter

iProX dataset URI