PXD056790-2

PXD056790 is an original dataset announced via ProteomeXchange.

Title	Remodeling of the neutrophil proteome upon exposure to Pseudomonas aeruginosa biofilms
Description	Neutrophils are the most prominent cell in the immune system, accounting for 70% of circulating leukocytes, and acting as the first line of defense against various invading pathogens. For example, neutrophils engage in phagocytosis, degranulation, and neutrophil extracellular trap formation to respond to pathogens. Pseudomonas aeruginosa is a Gram-negative bacterium that readily forms biofilms (i.e., high-density bacterial clusters attached to surfaces) to provide protection from the host immune system, antibiotic treatment, and antimicrobial agents. Neutrophils are essential to clear P. aeruginosa biofilms and infections through the actions of antimicrobial proteins and peptides. In this study, we evaluated proteome remodeling of the host following exposure of neutrophils (differentiated from HL-60 cells) to P. aeruginosa biofilms.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_07:01:58.130.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jennifer Geddes-McAlister
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Pseudomonas aeruginosa PAO1; NCBI TaxID: 208964;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 240

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-14 09:45:05	ID requested
1	2024-10-14 12:22:06	announced
⏵ 2	2024-10-22 07:01:58	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: neutrophils,Total proteome, Pseudomonas aeruginosa, biofilms

Dr. Jennifer Geddes-McAlister
contact affiliation	Molecular and Cellular Biology Department, University of Guelph, Guelph, ON, Canada
contact email	jgeddesm@uoguelph.ca
lab head
Jennifer Geddes-McAlister
contact affiliation	University of Guelph
contact email	jgeddesm@uoguelph.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/10/PXD056790

PRIDE project URI

• PRIDE
  1. PXD056790
     1. Label: PRIDE project
     2. Name: Remodeling of the neutrophil proteome upon exposure to Pseudomonas aeruginosa biofilms